caa a22 4500 477101704 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195195 doi (NATIONALLICENCE)springer-10.1007/BF00195195 The Brassica napus extA extensin gene is expressed in regions of the plant subject to tensile stresses [Elektronische Daten] [A. Shirsat, A. Bell, J. Spence, J. Harris] The expression of extA, an extensin gene from Brassica napus L. (oilseed rape) was examined in transgenic Nicotiana tabacum L. (tobacco) and untransformed Brassica juncea L. and B. napus tissues. Northern analysis showed that this gene maintained its normal pattern of expression when transferred to tobacco. In transgenic tobacco plants containing an extA promoter/β-glucuronidase coding sequence fusion, expression of extA was detected in the external and internal phloem of the main stem. High expression levels were seen in cortical parenchyma cells at the point where the axillary flowering branch joined the main stem. Expression was greatest in regions where the maximum tensile stress would seem to be exerted on the main stem by the weight of the axillary branch. It was confirmed that this expression pattern was due to tensile stress by using weights to induce expression of the fusion gene in axillary flowering stalks. In B. juncea pods, in-situ hybridisation studies showed that the extensin gene was strongly expressed in cells of the carpel walls within which considerable tensile stresses develop. Springer-Verlag, 1996 Brassica nationallicence Extensin nationallicence Nicotiana nationallicence Transgenic plants nationallicence Tensile stress nationallicence GUS : β-glucuronidase nationallicence X-GLUC : 5-bromo-4-chloro-3-indolyl glucuronide nationallicence SSC : saline sodium citrate nationallicence Shirsat A. School of Biological Sciences, University of Wales, LL57 2UW, Bangor, Gwynedd, UK aut Bell A. School of Biological Sciences, University of Wales, LL57 2UW, Bangor, Gwynedd, UK aut Spence J. aut Harris J. School of Biological Sciences, University of Durham, South Road, DH1 3LE, Durham, UK aut Planta Springer-Verlag 199/4(1996-08-01), 618-624 0032-0935 199:4<618 1996 199 425 https://doi.org/10.1007/BF00195195 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195195 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shirsat A. School of Biological Sciences, University of Wales, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Bell A. School of Biological Sciences, University of Wales, LL57 2UW, Bangor, Gwynedd, UK aut NATIONALLICENCE 700 1- Spence J. aut NATIONALLICENCE 700 1- Harris J. School of Biological Sciences, University of Durham, South Road, DH1 3LE, Durham, UK aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/4(1996-08-01), 618-624 0032-0935 199:4<618 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer