caa a22 4500 477101712 CHVBK 20180405111543.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195176 doi (NATIONALLICENCE)springer-10.1007/BF00195176 Regulation of the low-CO2-inducible polypeptides in Chlamydomonas reinhardtii [Elektronische Daten] [Arsenio Villarejo, Guillermo Reina, Ziyadin Ramazanov] Polypeptides of 21, 36 and 37 kDa are induced in the unicellular green alga Chlamydomonas reinhardtii Dang. when cells are transferred from high (2%) to low (0.03%) CO2 concentrations. The synthesis of these polypeptides is correlated with the induction of the CO2-concentrating mechanism. In this work we studied the effect of the growth conditions on the synthesis of these polypeptides with the aim of clarifying whether the induction of all three of these low-CO2-inducible polypeptides requires the same environmental factor. Our results showed that induction of the 21- and 36-kDa polypeptides under low-CO2 conditions occurred only in the light, while the 37-kDa periplasmic carbonic anhydrase (EC 4.2.1.1) was induced in light, darkness, and in both synchronous and asynchronous cultures. In addition, induction of these polypeptides appeared to be determined more by the O2/CO2 ratio than by the CO2 concentrations. None of these polypeptides could be induced in either of two different mutants of C. reinhardtii, one lacking ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) and the other with inactive enzyme. Our results indicate that the 21- and 36-kDa polypeptides are regulated by a mechanism different from that controlling the 37-kDa polypeptide. Springer-Verlag, 1996 Carbonic anhydrase nationallicence Chlamydomonas nationallicence CO2 concentrating mechanism nationallicence Light nationallicence Ribulose-1,5-bisphosphate carboxylase/oxygenase mutants nationallicence Protein synthesis nationallicence pCA : (periplasmic) carbonic anhydrase nationallicence Rubisco : ribulose-1,5-bisphosphate carboxylase/oxygenase nationallicence TAP : Trisacetate phosphate medium nationallicence Villarejo Arsenio Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain aut Reina Guillermo Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain aut Ramazanov Ziyadin Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain aut Planta Springer-Verlag 199/4(1996-08-01), 481-485 0032-0935 199:4<481 1996 199 425 https://doi.org/10.1007/BF00195176 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195176 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Villarejo Arsenio Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain aut NATIONALLICENCE 700 1- Reina Guillermo Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain aut NATIONALLICENCE 700 1- Ramazanov Ziyadin Institute of Applied Algology, Instituto Tecnológico de Canarias, Muelle de Taliarte s/n, E-35214, Telde, Canary Islands, Spain aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/4(1996-08-01), 481-485 0032-0935 199:4<481 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer