caa a22 4500 477101755 CHVBK 20180405111544.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195196 doi (NATIONALLICENCE)springer-10.1007/BF00195196 Jasmonate signalling can be uncoupled from abscisic acid signalling in barley: identification of jasmonate-regulated transcripts which are not induced by abscisic acid [Elektronische Daten] [Justin Lee, Benno Parthier, Marian Löbler] Jasmonate and abscisic acid induce several identical mRNAs and proteins in barley. In order to study whether both hormones act through the same signalling pathway, we identified four transcripts induced by jasmonic acid methylester (JM) in leaf segments of barley (Hordeum vulgare L. cv. Salome). These newly identified transcripts were not induced by abscisic acid within the tested times of 2-72 h. This finding supports the conclusion that jasmonate signalling in barley is independent of abscisic acid, in contrast to the wound-induction signal cascade of proteinase-inhibitor II in tomato and potato. Of the four isolated cDNAs, the putative translation frame of one was homologous to caffeic acid methyltransferase, another was homologous to chalcone synthase, and the C-terminus of the third showed homology to two proteins from rice (a salt-induced protein and a root-specific protein); the last cDNA was not homologous to any sequences in the databases. The new cDNAs will be valuable tools for studying jasmonate signal transduction in barley. Springer-Verlag, 1996 Abscisic acid nationallicence Hordeum nationallicence Jasmonic acid methylester nationallicence Jasmonate-regulated genes nationallicence Messenger RNA (differential display) nationallicence Signal transduction nationallicence ABA : cis-abscisic acid nationallicence CHS : chalcone synthase nationallicence JIP : jasmonate-induced protein nationallicence JM : jasmonic acid methylester nationallicence JRG : jasmonate-regulated gene nationallicence OMT : O-methyltransferase nationallicence ORF : open reading frame nationallicence PCR : polymerase chain reaction nationallicence Pin2 : oteinase inhibitor II nationallicence Rubisco : ribulose-1,5-bisphosphate carboxylase/oxygenase nationallicence Lee Justin Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany aut Parthier Benno Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany aut Löbler Marian Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany aut Planta Springer-Verlag 199/4(1996-08-01), 625-632 0032-0935 199:4<625 1996 199 425 https://doi.org/10.1007/BF00195196 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195196 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Justin Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany aut NATIONALLICENCE 700 1- Parthier Benno Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany aut NATIONALLICENCE 700 1- Löbler Marian Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle/Saale, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/4(1996-08-01), 625-632 0032-0935 199:4<625 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer