caa a22 4500 47710178X CHVBK 20180405111544.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195182 doi (NATIONALLICENCE)springer-10.1007/BF00195182 Regulation and molecular structure of a circadian oscillating protein located in the cell membrane of the prokaryote Synechococcus RF-1 [Elektronische Daten] [H- Chen, C- Chien, T- Huang] When a light/light-adapted culture of Synechococcus RF-1 is exposed to a diurnal light/dark regimen, the synthesis of more than ten of its polypeptides is known to become entrained to a circadian oscillating pattern which persists for some time under free-running conditions. One of the circadian oscillating polypeptides, COP23, was found to be located in the cell membrane. The rate of COP23 synthesis is controlled at the transcription level. In addition to the protein synthesis rate, the content of COP23 also exhibited a circadian rhythm. Pulse labeling with [35S]methionine revealed that COP23 was relatively stable in an arrhythmic culture. However, the exposure of Synechococcus RF-1 to a light/dark regimen induced not only a circadian synthesis rhythm, but also a rapid degradation of COP23 protein at a defined period of time. The induction of rapid protein degradation was prevented by the presence of chloramphenicol. The gene encoding the COP23 polypeptide has been cloned and sequenced. The amino acid sequence derived from the open-reading frame revealed that a signal peptide (28 amino acids) does not appear to be part of the mature COP23. The mature COP23 does not have a membrane-associated segment, and it is suggested to be a peripheral molecule. With respect to their DNA base sequence and protein amino acid sequence, none of the proteins documented in the EMBL and PC/Gene data bases are significantly homologous with the COP23 molecule. Springer-Verlag, 1996 Biological clock nationallicence Circadian rhythm nationallicence Molecular structure nationallicence Prokaryote nationallicence Protein (degradation, synthesis) nationallicence Synechococcus RF-1 nationallicence D : darkness nationallicence L : light nationallicence Chen H- Department of Biology, National Taiwan Normal University, Taipei, Taiwan 117, Republic of China aut Chien C- Department of Biology, National Taiwan Normal University, Taipei, Taiwan 117, Republic of China aut Huang T- Academia Sinica, Institute of Botany, Nankang, Taipei, Taiwan 115, Republic of China aut Planta Springer-Verlag 199/4(1996-08-01), 520-527 0032-0935 199:4<520 1996 199 425 https://doi.org/10.1007/BF00195182 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195182 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen H- Department of Biology, National Taiwan Normal University, Taipei, Taiwan 117, Republic of China aut NATIONALLICENCE 700 1- Chien C- Department of Biology, National Taiwan Normal University, Taipei, Taiwan 117, Republic of China aut NATIONALLICENCE 700 1- Huang T- Academia Sinica, Institute of Botany, Nankang, Taipei, Taiwan 115, Republic of China aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/4(1996-08-01), 520-527 0032-0935 199:4<520 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer