caa a22 4500 47710181X CHVBK 20180405111544.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195181 doi (NATIONALLICENCE)springer-10.1007/BF00195181 Disruption of an overlapping E-box/ABRE motif abolished high transcription of the napA storage-protein promoter in transgenic Brassica napus seeds [Elektronische Daten] [Kjell Stålberg, Mats Ellerstöm, Inès Ezcurra, Sergei Ablov, Lars Rask] The storage protein napin is one of the major protein components of Brassica napus L. (oilseed rape) seeds. To investigate the transcriptional regulation of the napin promoter, different constructs of the napin gene napA promoter were fused to the Escherichia coli uidA gene and transformed into B. napus. A -152-bp promoter construct directed a strong expression of the marker gene in mature seeds. The 5′ deletion of an additional 8 completely abolished this activity. This deletion disrupted sequence motifs that are similar to an E-box, (CA↓ NNTG) and an ABRE (CGCCA↓CGTGTCC) element (identity is indicated by bold face). Further, internal deletion of a segment corresponding to -133 to -121 caused an eightfold reduction in the activity of the -152 construct. This region contains an element, CAAACAC, conserved in many storage-protein gene promoters. These results imply that the E-box/ABRE-like sequence is a major motif of the napA promoter and suggest that the CAAACAC sequence is important for high activity of the napA promoter. Similar results have been obtained by analysing some of the constructs in transgenic tobacco, suggesting that many of the cis-elements in the napA promoter are conserved, at least in dicotyledonous species. Springer-Verlag, 1996 Brassica nationallicence Deletion analysis nationallicence Promoter nationallicence Seed nationallicence Storage protein nationallicence ABA : abscisic acid nationallicence GUS : glucuronidase nationallicence Stålberg Kjell Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Ellerstöm Mats Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Ezcurra Inès Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Ablov Sergei Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Rask Lars Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut Planta Springer-Verlag 199/4(1996-08-01), 515-519 0032-0935 199:4<515 1996 199 425 https://doi.org/10.1007/BF00195181 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195181 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stålberg Kjell Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Ellerstöm Mats Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Ezcurra Inès Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Ablov Sergei Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Rask Lars Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/4(1996-08-01), 515-519 0032-0935 199:4<515 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer