caa a22 4500 477101828 CHVBK 20180405111544.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00195184 doi (NATIONALLICENCE)springer-10.1007/BF00195184 Measurement of the electrical resistance of plasmodesmata and membranes of corn suspension-culture cells [Elektronische Daten] [Terena Holdaway-Clarke, N. Walker, Robyn Overall] Electrophysiological investigations of intercellular communication and membrane resistance in higher plants have been hampered by the difficulty in measuring these quantities independently. Uncertainty about the position of an electrode inserted into vacuolate tissue has further complicated such measurement. To overcome these problems sister cell pairs of a Zea mays L. ‘Black Mexican Sweet' suspension culture were used and dye was injected from the current-injecting electrode to determine the location of the electrode tip in each experiment. Of the impalements, 72% were cytoplasmic. The presence of plasmodesmata was fully incorporated into the electriccircuit model for the cell, and the resistance of the membrane of the current-injected cell was calculated, separate from the plasmodesmata resistance. This avoided some of the confusion resulting from work on multicellular tissue in which the position of the electrode and the extent of intercellular coupling is not determined. Using this technique, plasma-membrane resistivity was measured as 0.65 Ω·m2, the resistivity of the tonoplast and plasma membrane in series was 1.35 Ω·m2, and the resistance of a single plasmodesma was calculated to be 53 ± 11 GΩ. Springer-Verlag, 1996 Membrane resistivity nationallicence Microelectrode nationallicence Plasmodesmata nationallicence Suspension culture (electrophysiology) nationallicence Zea (electrophysiology) nationallicence BMS : Black Mexican Sweet nationallicence PD : potential difference nationallicence Rj : resistance of the plasmodesmata in the junction between cells nationallicence Rm : resistance of the plasma membrane of the current-injected cell nationallicence Rt : resistance of the tonoplast nationallicence V1, V2 : membrane PDs of sister cells nationallicence Holdaway-Clarke Terena School of Biological Sciences, A12, The University of Sydney, 2006, N.S.W, Australia aut Walker N. School of Biological Sciences, A12, The University of Sydney, 2006, N.S.W, Australia aut Overall Robyn School of Biological Sciences, A12, The University of Sydney, 2006, N.S.W, Australia aut Planta Springer-Verlag 199/4(1996-08-01), 537-544 0032-0935 199:4<537 1996 199 425 https://doi.org/10.1007/BF00195184 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195184 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holdaway-Clarke Terena School of Biological Sciences, A12, The University of Sydney, 2006, N.S.W, Australia aut NATIONALLICENCE 700 1- Walker N. School of Biological Sciences, A12, The University of Sydney, 2006, N.S.W, Australia aut NATIONALLICENCE 700 1- Overall Robyn School of Biological Sciences, A12, The University of Sydney, 2006, N.S.W, Australia aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/4(1996-08-01), 537-544 0032-0935 199:4<537 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer