caa a22 4500 477102018 CHVBK 20180405111545.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00196574 doi (NATIONALLICENCE)springer-10.1007/BF00196574 The expression of mRNAs for light-stress proteins in barley: Inverse relationship of mRNA levels of individual genes within the leaf gradient [Elektronische Daten] [Eyck Pötter, Jens Beator, Klaus Kloppstech] Two cDNAs coding for putative light-stress proteins of barley (Hordeum vulgare L.) were cloned and the expression of the corresponding mRNAs analyzed in the barley leaf and compared to that of the well-studied ELIP (early light-inducible protein) mRNA. During greening the mRNA for clone HL # 2, which shows homology to two rice proteins of as yet unknown function, was transiently induced; its level rose more slowly and remained elevated for a longer time than was described for ELIP mRNAs. The mRNA corresponding to clone HL # 13 was recognized as homologous to subunit P of pea glycine decarboxylase, a nuclear-encoded mitochondrial protein involved in photorespiration. Its mRNA level rose more slowly with cellular development than that of the mRNA for LHC II, the apoprotein of the chlorophyll-a/b-binding protein of PSII. The mRNAs of both novel proteins were induced by high light up to an irradiance of 2000 W·m−2. Their levels remained elevated under high light for up to 9 h, the longest time span examined, while after return to culture light conditions the mRNAs rapidly decayed, each with an individual time course. In green barley leaves the mRNA for clone HL # 2 was expressed to the highest level in the most basal segment, similar to that of ELIPs, while in contrast the mRNA for subunit P of glycine decarboxylase accumulated to the highest level in the leaf apex where the fully developed cells and mitochondria reside. The latter finding strongly indicates that photorespiration is regulated by high light also at the level of mRNA transcription or mRNA accumulation. In addition, we show that perception of light stress is under the control of cellular development and differentiation. Springer-Verlag, 1996 Chloroplast nationallicence Hordeum (leaf) nationallicence Light stress nationallicence Mitochondrion nationallicence Plant development nationallicence ABA : abscisic acid nationallicence ELIP : early light-inducible protein nationallicence GDC : glycine decarboxylase nationallicence HL : high light (2000 W·m−2) nationallicence LHC II : apoprotein of chlorophyll-a/b-binding protein of PSII nationallicence LL : low light (20 W·m−2) nationallicence P-GDC : P-subunit of GDC nationallicence Pötter Eyck Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany aut Beator Jens Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany aut Kloppstech Klaus Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany aut Planta Springer-Verlag 199/2(1996-06-01), 314-320 0032-0935 199:2<314 1996 199 425 https://doi.org/10.1007/BF00196574 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196574 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pötter Eyck Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany aut NATIONALLICENCE 700 1- Beator Jens Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany aut NATIONALLICENCE 700 1- Kloppstech Klaus Institut für Botanik, Universität Hannover, Herrenhäuser Strasse 2, D-30419, Hannover, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/2(1996-06-01), 314-320 0032-0935 199:2<314 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer