caa a22 4500 477102026 CHVBK 20180405111545.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00196569 doi (NATIONALLICENCE)springer-10.1007/BF00196569 Differential expression of plastome-encoded ndh genes in mesophyll and bundle-sheath chloroplasts of the C4 plant Sorghum bicolor indicates that the complex I-homologous NAD(P)H-plastoquinone oxidoreductase is involved in cyclic electron transport [Elektronische Daten] [Andreas Kubicki, Edgar Funk, Peter Westhoff, Klaus Steinmüller] Cyanobacteria and plastids harbor a putative NAD(P)H- or ferredoxin-plastoquinone oxidoreductase that is homologous to the NADH-ubiquinone oxidoreductase (complex I) of mitochondria and eubacteria. The enzyme is a multimeric protein complex that consists of at least 11 subunits (NDH-A-K) and is localized in the stroma lamellae of the thylakoid membrane system. We investigated the expression of the different subunits of the enzyme in mesophyll and bundle-sheath chloroplasts of Sorghum bicolor [L.] Moench, a C4 plant of the NADP-malic enzyme type. The relative amounts of the subunits NDH-H, -J and -K were strongly increased in bundle-sheath plastids as compared to mesophyll plastids. This increase was accompanied by enhanced transcript levels for all subunits except NDH-I. Because the main function of the protein complexes in the thylakoid membranes of bundle-sheath chloroplasts (photosystem I, cytochrome b 6/f-complex and ATPase) is the generation of ATP for CO2 fixation via cyclic electron transport, we conclude that the NAD(P)H/ferredoxin-plastoquinone oxidoreductase is an essential component of the cyclic electron-transport pathway in chloroplasts. Springer-Verlag, 1996 C4 photosynthesis nationallicence Cyclic electron transport nationallicence NAD(P)H-plastoquinone-oxidoreductase nationallicence Sorghum nationallicence Kubicki Andreas Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Funk Edgar Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Westhoff Peter Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Steinmüller Klaus Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut Planta Springer-Verlag 199/2(1996-06-01), 276-281 0032-0935 199:2<276 1996 199 425 https://doi.org/10.1007/BF00196569 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196569 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kubicki Andreas Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Funk Edgar Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Westhoff Peter Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Steinmüller Klaus Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstrasse 1, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/2(1996-06-01), 276-281 0032-0935 199:2<276 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer