caa a22 4500 477102042 CHVBK 20180405111545.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00196571 doi (NATIONALLICENCE)springer-10.1007/BF00196571 The regulation of NADPH-protochlorophyllide oxidoreductases A and B in green barley plants kept under a diurnal light/dark cycle [Elektronische Daten] [Hauke Holtorf, Klaus Apel] In etiolated barley (Hordeum vulgare L.) seedlings the light-induced accumulation of chlorophyll is controlled by two light-dependent NADPH-proto-chlorophyllide oxidoreductase (POR; EC 1.6.99.1) enzymes. While the concentration of one of these enzymes (POR A) and its mRNA rapidly decline during illumination, the second POR protein (POR B) and its mRNA remain at an approximately constant level during the transition from dark growth to the light. These results may suggest that only one of the enzymes, POR B, operates throughout the greening process and in light-adapted mature plants while the second enzyme, POR A, is active only in etiolated seedlings at the beginning of illumination. The fate of the two POR proteins and their mRNAs in fully green plants, however, has not been studied yet. In the present work we determined changes in the level of POR A and POR B proteins and mRNAs in green barley plants kept under a diurnal 12 h light/12 h dark cycle. In green barley plants, not only POR B is present but also trace amounts of POR A continue to reappear transiently at the end of a night period and seem to be involved in the synthesis and accumulation of chlorophyll at the beginning of each day. Springer-Verlag, 1996 Chlorophyll biosynthesis nationallicence Chloroplast development nationallicence Hordeum nationallicence Protochlorophyllide oxidoreductase nationallicence Chl : chlorophyll nationallicence Chlide : chlorophyllide nationallicence Lhcb : light-harvesting chlorophyll a/b protein nationallicence Pchlide : protochlorophyllide nationallicence POR : NADPH-protochlorophyllide oxidoreductase nationallicence Holtorf Hauke Institut für Pflanzenwissenschaften, ETH-Zentrum, Swiss Federal Institute of Technology Zürich (ETH), Universitätstrasse 2, CH-8092, Zürich, Switzerland aut Apel Klaus Institut für Pflanzenwissenschaften, ETH-Zentrum, Swiss Federal Institute of Technology Zürich (ETH), Universitätstrasse 2, CH-8092, Zürich, Switzerland aut Planta Springer-Verlag 199/2(1996-06-01), 289-295 0032-0935 199:2<289 1996 199 425 https://doi.org/10.1007/BF00196571 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196571 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holtorf Hauke Institut für Pflanzenwissenschaften, ETH-Zentrum, Swiss Federal Institute of Technology Zürich (ETH), Universitätstrasse 2, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Apel Klaus Institut für Pflanzenwissenschaften, ETH-Zentrum, Swiss Federal Institute of Technology Zürich (ETH), Universitätstrasse 2, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/2(1996-06-01), 289-295 0032-0935 199:2<289 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer