caa a22 4500 477102050 CHVBK 20180405111545.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00196558 doi (NATIONALLICENCE)springer-10.1007/BF00196558 Different ratios of sucrose/raffinose-induced membrane depolarizations in the mesophyll of species with symplasmic ( Catharanthus roseus, Ocimum basilicum ) or apoplasmic ( Impatiens walleriana, Vicia faba ) minor-vein configurations [Elektronische Daten] [Aart van Bel, Janneke Hendriks, Eline Boon, Yuri Gamalei, Arjan van de Merwe] In mesophyll cells of species with a symplasmic (Ocimum basilicum, Catharanthus roseus, Magnolia denudata) or an apoplasmic (Vicia faba, Impatiens walleriana, Bellis perennis) minor-vein configuration, membrane depolarizations in response to 20 or 200 mol·m−3 raffinose and sucrose were measured. Ageing period and resting potential marginally affected the degree of depolarization. The symplasmic species showed similar depolarization responses to 20 and 200 mol·m−3 sucrose or raffinose. In the apoplasmic species, depolarization increased statistically significantly from 20 to 200 mol·m−3 sucrose, whereas the depolarization response to raffinose was equal at both concentrations. In the apoplasmic species, moreover, the depolarization response to raffinose was significantly weaker than to sucrose at all concentrations. A major difference between symplasmic and apoplasmic species seems to lie in the scantiness of raffinose carriers in the mesophyll plasma membrane of species with the apoplasmic mode of phloem loading. Springer-Verlag, 1996 Galactosyl oligosaccharides nationallicence Membrane depolarization nationallicence Phloem loading (apoplasmic, symplasmic) nationallicence Raffinose uptake nationallicence Sucrose uptake nationallicence 20R(200R) : 20(200) mol·m−3 raffinose nationallicence 20S(200S) : 20(200) mol·m−3 sucrose nationallicence van Bel Aart Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut Hendriks Janneke Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut Boon Eline Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut Gamalei Yuri Department of Ecophysiology, Komarov Botanical Institute, 2 Professor Popov Street, 197376, St. Petersburg, Russia aut van de Merwe Arjan Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut Planta Springer-Verlag 199/2(1996-06-01), 185-192 0032-0935 199:2<185 1996 199 425 https://doi.org/10.1007/BF00196558 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196558 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Bel Aart Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Hendriks Janneke Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Boon Eline Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Gamalei Yuri Department of Ecophysiology, Komarov Botanical Institute, 2 Professor Popov Street, 197376, St. Petersburg, Russia aut NATIONALLICENCE 700 1- van de Merwe Arjan Department of Plant Ecology and Evolutionary Biology, Transport Physiology Research Group, Sorbonnelaan 16, NL-3584 CA, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/2(1996-06-01), 185-192 0032-0935 199:2<185 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer