caa a22 4500 477102077 CHVBK 20180405111545.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00196566 doi (NATIONALLICENCE)springer-10.1007/BF00196566 Localization of pectins and arabinogalactan-proteins in lily ( Lilium longiflorum L.) pollen tube and style, and their possible roles in pollination [Elektronische Daten] [Guang Jauh, Elizabeth Lord] In lily, adhesion of the pollen tube to the transmitting-tract epidermal cells (TTEs) is purported to facilitate the effective movement of the tube cell to the ovary. In this study, we examine the components of the extracellular matrices (ECMs) of the lily pollen tubes and TTEs that may be involved in this adhesion event. Several monoclonal antibodies to plant cell wall components such as esterified pectins, unesterified pectins, and arabinogalactan-proteins (AGPs) were used to localize these molecules in the lily pollen tube and style at both light microscope (LM) and transmission electron microscope (TEM) levels. In addition, (β-d-Glc)3 Yariv reagent which binds to AGPs was used to detect AGPs in the pollen tube and style. At the LM level, unesterified pectins were localized to the entire wall in in-vivo- and in-vitro-grown pollen tubes as well as to the surface of the stylar TTEs. Esterified pectins occurred at the tube tip region (with some differences in extent in in-vivo versus in-vitro tubes) and were evenly distributed in the entire style. At the TEM level, esterified pectins were detected inside pollen tube cell vesicles and unesterified pectins were localized to the pollen tube wall. The in-vivo pollen tubes adhere to each other and can be separated by pectinase treatment. At the LM level, AGP localization occurred in the tube tip of both in-vivo- and in-vitro-grown pollen tubes and, in the case of one AGP probe, on the surface of the TTEs. Another AGP probe localized to every cell of the style except the surface of the TTE. At the TEM level, AGPs were mainly found on the plasma membrane and vesicle membranes of in-vivo-grown pollen tubes as well as on the TTE surface, with some localization to the adhesion zone between pollen tubes and style. (β-d-Glc)3 Yariv reagent bound to the in-vitro-grown pollen tube tip and significantly reduced the growth of both in-vitro- and in-vivo-grown pollen tubes. This led to abnormal expansion of the tube tip and random deposition of callose. These effects could be overcome by removal of (β-d-Glc)3 Yariv reagent which resulted in new tube tip growth zones emerging from the flanks of the arrested tube tip. The possible roles of pectins and AGPs in adhesion during pollination and pollen tube growth are discussed. Springer-Verlag, 1996 Arabinogalactan-protein (MAb JIM 13-16, MAC207, LM2) nationallicence Immunolocalization nationallicence Lilium (pollen tube) nationallicence Pectin (MAb JIM5, JIM7) nationallicence Pollen tube nationallicence (β- d -Glc)3 Yariv reagent nationallicence AGP : arabinogalactan-protein nationallicence ECM : extracellular matrix nationallicence Glc : glucose nationallicence MAbs : monoclonal antibodies nationallicence LM : light microscope nationallicence Man : mannose nationallicence TEM : transmission electron microscope nationallicence TTE : transmitting tract epidermal cell nationallicence Jauh Guang Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Lord Elizabeth Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut Planta Springer-Verlag 199/2(1996-06-01), 251-261 0032-0935 199:2<251 1996 199 425 https://doi.org/10.1007/BF00196566 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196566 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jauh Guang Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 700 1- Lord Elizabeth Department of Botany and Plant Sciences, University of California, 92521-0124, Riverside, CA, USA aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/2(1996-06-01), 251-261 0032-0935 199:2<251 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer