caa a22 4500 477102085 CHVBK 20180405111545.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00196561 doi (NATIONALLICENCE)springer-10.1007/BF00196561 Starch debranching enzyme (R-enzyme or pullulanase) from developing rice endosperm: purification, cDNA and chromosomal localization of the gene [Elektronische Daten] [Yasunori Nakamura, Takayuki Umemoto, Naoki Ogata, Yoshihide Kuboki, Masahiro Yano, Takuji Sasaki] Starch debranching enzyme (R-enzyme or pullulanase) was purified to homogeneity from developing endosperm of rice (Oryza sativa L. cv. Fujihikari) using a variety of high-performance liquid chromatography columns, and characterized. A cDNA clone encoding the full length of the rice endosperm debranching enzyme was isolated and its nucleotide sequence was determined. The cDNA contains an open reading frame of 2958 bp. The mature debranching enzyme of rice appears to be composed of 912 amino acids with a predicted relative molecular mass (Mr) of 102069 Da, similar in size to its Mr of about 100 000 Da estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The amino acid sequence of rice debranching enzyme is substantially similar to that of bacterial pullulanase, while it bears little similarity to that of bacterial isoamylase or to glycogen debranching enzymes from human muscle and rabbit muscle. Southern blot analyses strongly suggest that the debranching enzyme gene is present as a single copy in the rice genome. Analysis by restriction fragment length polymorphism with a probe including the 3′-untranslated region of cDNA for rice debranching enzyme confirmed that the debranching enzyme gene is located on chromosome 4. Springer-Verlag, 1996 Amylopectin nationallicence Debranching enzyme nationallicence Glycogen nationallicence Oryza endosperm nationallicence Pullulanase nationallicence R-enzyme nationallicence Starch nationallicence RFLP : restriction fragment length polymorphism nationallicence SDS-PAGE : polyacrylamide gel electrophoresis in sodium dodecyl sulfate nationallicence Nakamura Yasunori Department of Applied Physiology, National Institute of Agrobiological Resources (NIAR), Kannondai, Tsukuba, 305, Ibaraki, Japan aut Umemoto Takayuki Department of Lowland Farming, Tohoku National Agricultural Experiment Station, Yotsuya, Omagari, 014-01, Akita, Japan aut Ogata Naoki Department of Applied Physiology, National Institute of Agrobiological Resources (NIAR), Kannondai, Tsukuba, 305, Ibaraki, Japan aut Kuboki Yoshihide Rice Genome Research Program, NIAR/STAFF, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan aut Yano Masahiro Rice Genome Research Program, NIAR/STAFF, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan aut Sasaki Takuji Rice Genome Research Program, NIAR/STAFF, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan aut Planta Springer-Verlag 199/2(1996-06-01), 209-218 0032-0935 199:2<209 1996 199 425 https://doi.org/10.1007/BF00196561 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00196561 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Yasunori Department of Applied Physiology, National Institute of Agrobiological Resources (NIAR), Kannondai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Umemoto Takayuki Department of Lowland Farming, Tohoku National Agricultural Experiment Station, Yotsuya, Omagari, 014-01, Akita, Japan aut NATIONALLICENCE 700 1- Ogata Naoki Department of Applied Physiology, National Institute of Agrobiological Resources (NIAR), Kannondai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kuboki Yoshihide Rice Genome Research Program, NIAR/STAFF, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Yano Masahiro Rice Genome Research Program, NIAR/STAFF, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sasaki Takuji Rice Genome Research Program, NIAR/STAFF, Kamiyokoba, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Planta Springer-Verlag 199/2(1996-06-01), 209-218 0032-0935 199:2<209 1996 199 425 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer