caa a22 4500 477105114 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01574693 doi (NATIONALLICENCE)springer-10.1007/BF01574693 Molecular evidence for the presence of novel actinomycete lineages in a temperate forest soil [Elektronische Daten] [H McVeigh, J Munro, T Embley] PCR primers were designed to selectively recover partial (∼1100 bp) actinomycete 16S ribosomal DNA sequences from a temperate forest soil. A gene library was made and colony PCR was used to identify clones containing inserts. Unique clones were identified and partial or complete insert sequences were determined for 53 clones. Phylogenetic analyses revealed that 46 (87%) of the clones sampled contained 16S rDNA sequences which fell within the actinomycete radiation. The largest group of 34 sequences formed two closely related monophyletic groups in the 16S rRNA tree, which in turn formed a weakly supported sister group with the sequence fromActinomadura madurae. Four novel 16S rDNA lineages were detected inMycobacterium, one inPropionibacterium and one inCorynebacterium. Three novel sequences weakly grouped withSporichthya polymorpha. Two sequences formed an isolated lineage not closely related to any of the reference actinomycetes. Our results lend strong support to the hypothesis that cultured (and sequenced) actinomycetes do not adequately describe the diversity of this group in the environment. Society for Industrial Microbiology, 1996 actinomycete diversity nationallicence 16S rDNA sequences nationallicence non-culture methods nationallicence phylogenetic analysis nationallicence novel lineages nationallicence McVeigh H. Microbiology Division, The Natural History Museum, Cromwell Road, SW7 5BD, London aut Munro J. Compound Diversity, GlaxoWellcome Medicines Research Centre, Gunnels Wood Road, SG1 2NY, Stevenage, Hertfordshire, UK aut Embley T. Microbiology Division, The Natural History Museum, Cromwell Road, SW7 5BD, London aut Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 197-204 0169-4146 17:3-4<197 1996 17 10295 https://doi.org/10.1007/BF01574693 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574693 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McVeigh H. Microbiology Division, The Natural History Museum, Cromwell Road, SW7 5BD, London aut NATIONALLICENCE 700 1- Munro J. Compound Diversity, GlaxoWellcome Medicines Research Centre, Gunnels Wood Road, SG1 2NY, Stevenage, Hertfordshire, UK aut NATIONALLICENCE 700 1- Embley T. Microbiology Division, The Natural History Museum, Cromwell Road, SW7 5BD, London aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 197-204 0169-4146 17:3-4<197 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer