caa a22 4500 477105165 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01574691 doi (NATIONALLICENCE)springer-10.1007/BF01574691 Genetic diversity of soil microorganisms assessed by analysis of hsp70 (dnaK) sequences [Elektronische Daten] [W Yap, X Li, T Soong, J Davies] The genetic diversity of a soil microbial community was assessed by analysis of clonedhsp70 sequences. A clone library was generated by polymerase chain reaction-mediated amplification of a 650-base pair fragment of thehsp70 gene, using DNA extracted from soil, without culturing the microorganisms. Fifty-five random clones were sequenced and their amino acid sequences deduced. Analysis of the amino acid sequence of the clones revealed the presence of signature sequences in common with known prokaryotic and lower eukaryotic HSP70 homologs. None of the 55 analyzed sequences were identical to each other or to a published sequence. These results confirm the presence of considerable genetic diversity within soil microbial communities, the major proportion of which remains uncharacterized. Society for Industrial Microbiology, 1996 hsp70 nationallicence soil microorganisms nationallicence soil DNA nationallicence microbial diversity nationallicence Yap W. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut Li X. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut Soong T. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut Davies J. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 179-184 0169-4146 17:3-4<179 1996 17 10295 https://doi.org/10.1007/BF01574691 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574691 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yap W. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Li X. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Soong T. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Davies J. West-East Centre for Microbial Diversity, BC Research Building, 3650 Wesbrook Mall, V6S 2L2, Vancouver, BC, Canada aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 179-184 0169-4146 17:3-4<179 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer