caa a22 4500 477105203 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01574692 doi (NATIONALLICENCE)springer-10.1007/BF01574692 Quantitative comparisons of in situ microbial biodiversity by signature biomarker analysis [Elektronische Daten] [DC White, JO Stair, DB Ringelberg] Microscopic examinations have convinced microbial ecologists that the culturable microbes recovered from environmental samples represent a tiny proportion of the extant microbiota. Methods for recovery and enzymatic amplification of nucleic acids from environmental samples have shown that a huge diversity existsin situ, far exceeding any expectations which were based on direct microscopy. It is now theoretically possible to extract, amplify and sequence all the nucleic acids from a community and thereby gain a comprehensive measure of the diversity as well as some insights into the phylogeny of the various elements within this community. Unfortunately, this analysis becomes economically prohibitive if applied to the multitude of niches in a single biome let alone to a diverse set of environments. It is also difficult to utilize PCR amplification on nucleic acids from some biomes because of coextracting enzymatic inhibitors. Signature biomarker analysis which potentially combines gene probe and lipid analysis on the same sample, can serve as a complement to massive environmental genome analysis in providing quantitative comparisons between microniches in the biome under study. This analysis can also give indications of the magnitude of differences in biodiversity in the blome as well as provide insight into the phenotypic activities of each community in a rapid and cost-effective manner. Applications of signature lipid biomarker analysis to define quantitatively the microbial viable biomass of portions of an Eastern USA deciduous forest, are presented. Society for Industrial Microbiology, 1996 microbial biodiversity nationallicence signature lipid biomarkers (SLB) nationallicence phospholipid fatty acids (PLFA) nationallicence in situ microbial biomass nationallicence community composition nationallicence nutritional/physiological status nationallicence non-culturable viable biomass nationallicence White DC Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, 37932-2575, Knoxville, TN, USA aut Stair JO Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, 37932-2575, Knoxville, TN, USA aut Ringelberg DB Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, 37932-2575, Knoxville, TN, USA aut Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 185-196 0169-4146 17:3-4<185 1996 17 10295 https://doi.org/10.1007/BF01574692 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574692 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- White DC Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, 37932-2575, Knoxville, TN, USA aut NATIONALLICENCE 700 1- Stair JO Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, 37932-2575, Knoxville, TN, USA aut NATIONALLICENCE 700 1- Ringelberg DB Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, 37932-2575, Knoxville, TN, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 185-196 0169-4146 17:3-4<185 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer