caa a22 4500 477105211 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01574700 doi (NATIONALLICENCE)springer-10.1007/BF01574700 Isolation of zoosporogenous actinomycetes from desert soils [Elektronische Daten] [G Garrity, BK Heimbuch, M Gagliardi] We have undertaken a study to estimate the species diversity of zoosporogenous actinomycetes that can be isolated from an arid environment. The study site encompassed an area of approximately 22 000 square kilometers of the Mojave Desert along the California-Nevada border. A series of 29 soil samples was collected along two intersecting transects of approximately 190 and 240 km which traversed a number of distinct ecosystems. A0 horizon soils were collected from the rhizosphere of the predominant vegetation at each sampling site and screened for the target genera using selective isolation techniques: chemoattraction (xylose and γ-collidine) and baiting with hair. Following incubation of primary isolation plates for 28 days at 28°C, all colonies that exhibited filamentous growth, presence of sporangia and/or motile spores upon direct microscopic observation (450 and 1000×) were further characterized by fatty acid analysis (FAME). Most of the isolates fell into three broad clusters that roughly correlated with presumptive genus assignments. Individual isolates could be assigned to 226 FAME biotypes based on chromatographic similarity (≥85%). The dominant species (514/826 isolates) belong to a previously undescribed taxon that morphologically resemblesGeodermatophilus but possesses unique FAME profiles that include at least three novel lipids. The remainder of the isolates were species ofActinoplanes, indeterminate species or vagrant isolates ofStreptomyces. Society for Industrial Microbiology, 1996 Actinoplanes nationallicence actinomycetes nationallicence desert nationallicence ecology nationallicence FAME nationallicence fatty-acid analysis nationallicence Geodermatophilus nationallicence zoospores nationallicence Garrity G. Natural Products Discovery and Development, Merck Research Laboratories, R80Y-300, Box 2000, 07065-0900, Rahway, NJ, USA aut Heimbuch BK Natural Products Discovery and Development, Merck Research Laboratories, R80Y-300, Box 2000, 07065-0900, Rahway, NJ, USA aut Gagliardi M. Natural Products Discovery and Development, Merck Research Laboratories, R80Y-300, Box 2000, 07065-0900, Rahway, NJ, USA aut Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 260-267 0169-4146 17:3-4<260 1996 17 10295 https://doi.org/10.1007/BF01574700 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574700 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Garrity G. Natural Products Discovery and Development, Merck Research Laboratories, R80Y-300, Box 2000, 07065-0900, Rahway, NJ, USA aut NATIONALLICENCE 700 1- Heimbuch BK Natural Products Discovery and Development, Merck Research Laboratories, R80Y-300, Box 2000, 07065-0900, Rahway, NJ, USA aut NATIONALLICENCE 700 1- Gagliardi M. Natural Products Discovery and Development, Merck Research Laboratories, R80Y-300, Box 2000, 07065-0900, Rahway, NJ, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 260-267 0169-4146 17:3-4<260 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer