caa a22 4500 477105238 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01574696 doi (NATIONALLICENCE)springer-10.1007/BF01574696 Diversity among bacteria isolated from the deep subsurface [Elektronische Daten] [IV Zlatkin, M Schneider, FJ de Bruijn, LJ Forney] Culturable bacteria from the deep subsurface (179 m) at Cerro Negro, New Mexico were isolated and characterized. The average number of viable aerobic bacteria was estimated to be 5×105g−1 of sediment, but only about 0.1% of these could be recovered on agar medium when incubated under aerobic conditions. Of 158 strains isolated from this depth, 92 were characterized by cellular fatty acid profiles (FAME), 36 by analysis of partial 16S rDNA sequences, and 44 by rep-PCR genome fingerprint analysis using three different sets of oligonucleotide primers (REP, BOX, or ERIC). These analyses showed the majority of isolates (67%) were Gram-positive bacteria and primarily members of genera with a high %G+C DNA. The remaining isolates were α-subdivisionProteobacteria (19%) and members of the flavobacteria group (14%). The diversity indices based on these different methods of characterization were very high suggesting this subsurface habitat harbors a highly diverse microbial community. Society for Industrial Microbiology, 1996 heterotrophic bacteria nationallicence diversity nationallicence deep subsurface nationallicence 16S rDNA nationallicence FAME nationallicence rep-PCR nationallicence Zlatkin IV Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut Schneider M. Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut de Bruijn FJ Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut Forney LJ Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 219-227 0169-4146 17:3-4<219 1996 17 10295 https://doi.org/10.1007/BF01574696 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574696 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zlatkin IV Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Schneider M. Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- de Bruijn FJ Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Forney LJ Center for Microbial Ecology, 540 Plant and Soil Sciences, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 219-227 0169-4146 17:3-4<219 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer