caa a22 4500 477105254 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01574698 doi (NATIONALLICENCE)springer-10.1007/BF01574698 Physiologic, morphologic and behavioral responses of perpetual cultures of Caulobacter crescentus to carbon, nitrogen and phosphorus limitations [Elektronische Daten] [E Felzenberg, G Yang, J Hagenzieker, J Poindexter] Perpetual cultures ofCaulobacter crescentus were maintained in a chemostat and provided with media that provided glucose, glutamate, ammonium chloride, and Na/K phosphate as sources, respectively, of C, of C and N, of N and of P. All cultures were maintained at the same relative flow rate, f/V, so that reproductive rate was constant. As the C∶N∶P ratio was shifted from a balanced ratio of 100 μg C∶9-10 μg N∶1 μg P, the cells modulated their rates of uptake, reducing the rates of uptake of excess element-sources and accelerating the uptake of potential sources of the limiting element. The change in N-limited cells was the greatest, resulting in a nearly 150-fold increase in rate of uptake of amino acids. Poly-β-hydroxybutyrate or polyphosphate was stored whenever C or P, respectively, was not the limiting element. Direct measurement of cell and stalk surface areas on purified peptidoglycan sacculi of P-limited cells revealed that the surface of both swarmer and stalked cells, as well as the stalk surface, participated in the accelerated rate of phosphate uptake. Swarmers from N-limited populations were the only cells that exhibited chemotactic responsiveness-to methionine, NH4Cl and glutamate, but not to glucose or phosphate-sufficient to be detectable in a microcapillary assay. In populations that were C-limited and provided with ammonium ions as the principal source of N, morphogenesis and reproduction deteriorated and a steady state could not be maintained. Generally, the responses ofC. crescentus were appropriate to manage unbalanced nutrient supplies, but this oligoheterotrophic organism did not tolerate excesses of inorganic nutrients when limited for its sources of C, of energy, and of ammonium acceptor. Society for Industrial Microbiology, 1996 C∶N∶P ratio nationallicence Caulobacter crescentus nationallicence cell surface area nationallicence chemostat nationallicence chemotaxis nationallicence uptake nationallicence Felzenberg E. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut Yang G. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut Hagenzieker J. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut Poindexter J. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 235-252 0169-4146 17:3-4<235 1996 17 10295 https://doi.org/10.1007/BF01574698 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574698 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Felzenberg E. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut NATIONALLICENCE 700 1- Yang G. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut NATIONALLICENCE 700 1- Hagenzieker J. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut NATIONALLICENCE 700 1- Poindexter J. Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, 10027-6598, New York, NY, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/3-4(1996-09-01), 235-252 0169-4146 17:3-4<235 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer