caa a22 4500 477105300 CHVBK 20180405111551.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01570144 doi (NATIONALLICENCE)springer-10.1007/BF01570144 A structured approach to design and operation of biotransformation processes [Elektronische Daten] [M Lilly, J Woodley] A structured approach to design and operation of biotransformation (bioconversion) processes, based on previous case studies, has been developed. This requires knowledge of the key characteristics of a biotransformation which determine the constraints on process selection. The approach is illustrated for five biotransformations, two enzymic and three microbial. Some generic problems such as low water solubility and volatility of reactants, reactant and product toxicity have been identified. The microblal oxidations of toluene and fluorobenzene to toluenecis-glycol and fluorocatechol respectively byPseudomonas putida have been used to illustrate how these constraints may be overcome by addition of tetradecane as a second liquid phase, use of a membrane oxygenator and introduction ofin situ product removal. Society for Industrial Microbiology, 1996 biotransformation nationallicence aromatic oxidation nationallicence process selection nationallicence Pseudomonas putida nationallicence Lilly M. The Advanced Centre for Biochemical Engineering, University College London, London, UK aut Woodley J. The Advanced Centre for Biochemical Engineering, University College London, London, UK aut Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 24-29 0169-4146 17:1<24 1996 17 10295 https://doi.org/10.1007/BF01570144 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570144 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lilly M. The Advanced Centre for Biochemical Engineering, University College London, London, UK aut NATIONALLICENCE 700 1- Woodley J. The Advanced Centre for Biochemical Engineering, University College London, London, UK aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 24-29 0169-4146 17:1<24 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer