caa a22 4500 477105327 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01570147 doi (NATIONALLICENCE)springer-10.1007/BF01570147 Expression of mouse metallothionein in the cyanobacterium Synechococcus PCC7942 [Elektronische Daten] [J Erbe, K Taylor, L Hall] A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942. Society for Industrial Microbiology, 1996 metallothionein nationallicence cyanobacteria nationallicence metals nationallicence bioremediation nationallicence Erbe J. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut Taylor K. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut Hall L. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 41-46 0169-4146 17:1<41 1996 17 10295 https://doi.org/10.1007/BF01570147 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570147 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erbe J. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut NATIONALLICENCE 700 1- Taylor K. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut NATIONALLICENCE 700 1- Hall L. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 41-46 0169-4146 17:1<41 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer