caa a22 4500 477105394 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01570148 doi (NATIONALLICENCE)springer-10.1007/BF01570148 A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli [Elektronische Daten] [G Gosset, J Yong-Xiao, A Berry] Different approaches to increasing carbon commitment to aromatic amino acid biosynthesis were compared in isogenic strains ofEscherichia coli. In a strain having a wild-type PEP: glucose phosphotransferase (PTS) system, inactivation of the genes encoding pyruvate kinase (pykA andpykF) resulted in a 3.4-fold increase in carbon flow to aromatic biosynthesis. In a strain already having increased carbon flow to aromatics by virtue of overexpression of thetktA gene (encoding transketolase), thepykA and/orpykF mutations had no effect. A PTS glucose+ mutant showed a 1.6-fold increase in carbon flow to aromatics compared to the PTS+ control strain. In the PTS− glucose+ host background, overexpression oftktA caused a further 3.7-fold increase in carbon flow, while inactivation ofpykA andpykF caused a 5.8-fold increase. When all of the variables tested (PTS− glucose+,pykA, pykF, and overexpressedtktA) were combined in a single strain, a 19.9-fold increase in carbon commitment to aromatic biosynthesis was achieved. Society for Industrial Microbiology, 1996 amino acids nationallicence aromatics nationallicence E. coli nationallicence DAHP nationallicence PEP nationallicence Gosset G. Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250, Cuernavaca, Morelos, México aut Yong-Xiao J. Genencor International, 925 Page Mill Road, 94304-1013, Palo Alto, CA, USA aut Berry A. Genencor International, 925 Page Mill Road, 94304-1013, Palo Alto, CA, USA aut Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 47-52 0169-4146 17:1<47 1996 17 10295 https://doi.org/10.1007/BF01570148 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570148 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gosset G. Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250, Cuernavaca, Morelos, México aut NATIONALLICENCE 700 1- Yong-Xiao J. Genencor International, 925 Page Mill Road, 94304-1013, Palo Alto, CA, USA aut NATIONALLICENCE 700 1- Berry A. Genencor International, 925 Page Mill Road, 94304-1013, Palo Alto, CA, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 47-52 0169-4146 17:1<47 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer