caa a22 4500 477105408 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01570140 doi (NATIONALLICENCE)springer-10.1007/BF01570140 Variation in sessile microflora during biofilm formation on AISI-304 stainless steel coupons [Elektronische Daten] [F de França, M Lutterbach] Coupons of stainless steel type AISI-304 were exposed to the industrial cooling system of a petrochemical plant fed by seawater from the Guanabara Bay, Rio de Janeiro, Brazil, in order to study thein situ formation of biofilms. Bacteria, microalgae and fungi were detected on the coupons as soon as 48 h after exposure. Their respective numbers were determined at times 48, 96 and 192 h and over the following 8 weeks. Aerobic, anaerobic and sulfate-reducing bacteria were quantified according to the technique of the most probable number, and fungi by the pour plate technique. The number of microorganisms present in the forming biofilm varied over the experimental period, reaching maximal levels of 14×1011 cells cm−2, 30×1013 cells cm−2, 38×1011 cells cm−2 and 63×105 cells cm−2, respectively, for aerobic bacteria, anaerobic bacteria, sulfate-reducing bacteria and fungi, and the dynamics of this variation depended on the group of microorganisms.Bacillus sp,Escherichia coli, Serratia sp andPseudomonas putrefaciens were identified among the aerobic bacteria isolated. Additionally, microalgae and bacteria of the genusGallionella were also detected. Nonetheless, no evidence of corrosion was found on the stainless steel type AISI-304 coupons over the experimental period. Society for Industrial Microbiology, 1996 biofilm nationallicence cooling water nationallicence microbiologically influenced corrosion nationallicence microbial fouling nationallicence stainless steel nationallicence de França F. Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro (UFRJ) Centro de Tecnologia, Bloco E, Ilha do Fundäo, 21941-900, Rio de Janeiro, RJ aut Lutterbach M. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), 21941-900, RJ, Brazil aut Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 6-10 0169-4146 17:1<6 1996 17 10295 https://doi.org/10.1007/BF01570140 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570140 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de França F. Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro (UFRJ) Centro de Tecnologia, Bloco E, Ilha do Fundäo, 21941-900, Rio de Janeiro, RJ aut NATIONALLICENCE 700 1- Lutterbach M. Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), 21941-900, RJ, Brazil aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/1(1996-07-01), 6-10 0169-4146 17:1<6 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer