caa a22 4500 477105556 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF01574767 doi (NATIONALLICENCE)springer-10.1007/BF01574767 Intraspecific diversity in the fungal species Chaunopycnis alba [Elektronische Daten] Implications for microbial screening programs [C Möller, G Weber, MM Dreyfuss] Intraspecific variation among 84 isolates of the anamorphic fungusChaunopycnis alba from 26 different geographical locations was analyzed by investigating optimal growth temperatures, differences in the production of secondary metabolites and presence or absence of the cyclosporin synthetase gene. The genetic diversity was assessed using random amplified polymorphic DNA (RAPD). Analysis of these data showed high genetic, metabolic and physiological diversity within this species. Isolates from the Antarctic represented the most homogeneous group withinC. alba and together with isolates from the Arctic these polar strains differed from alpine, temperate and tropical strains by low optimal growth temperatures and by low production of secondary metabolites. Isolates from tropical climes were characterized by high optimal growth temperatures and by the production of comparatively diverse metabolite spectra. Most of the isolates that were similar in the combination of their physiological and metabolic characters were also genetically related. Isolates from different geographical origins did not show many similarities, with the exception of the cyclosporin A-producing isolates, and large diversity could be observed even within a single habitat. This leads us to the suggestion that for pharmaceutical screening programs samples should be collected from a diversity of different geographical and climatic locations. For the selection of strains for screening the RAPD assay seems to be the most powerful tool. It reflected the highest intraspecific diversity and the results corresponded well with the other characteristics. Society for Industrial Microbiology, 1996 intraspecific variation nationallicence RAPD nationallicence HPLC profile nationallicence temperature requirements nationallicence cyclosporin synthetase gene nationallicence creativity nationallicence Möller C. Preclinical Research SANDOZ Pharma Ltd, LFU, PKF, Bldg 506/413, CH-4002, Basel, Switzerland aut Weber G. Biochemie GmbH, A-6330, Kufstein-Schaftenau, Austria aut Dreyfuss MM Preclinical Research SANDOZ Pharma Ltd, LFU, PKF, Bldg 506/413, CH-4002, Basel, Switzerland aut Journal of Industrial Microbiology Springer-Verlag 17/5-6(1996-11-01), 359-372 0169-4146 17:5-6<359 1996 17 10295 https://doi.org/10.1007/BF01574767 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01574767 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Möller C. Preclinical Research SANDOZ Pharma Ltd, LFU, PKF, Bldg 506/413, CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Weber G. Biochemie GmbH, A-6330, Kufstein-Schaftenau, Austria aut NATIONALLICENCE 700 1- Dreyfuss MM Preclinical Research SANDOZ Pharma Ltd, LFU, PKF, Bldg 506/413, CH-4002, Basel, Switzerland aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/5-6(1996-11-01), 359-372 0169-4146 17:5-6<359 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer