caa a22 4500 477105645 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01570052 doi (NATIONALLICENCE)springer-10.1007/BF01570052 d -Glucose does not catabolite repress a transketolase-deficient d -ribose-producing Bacillus subtilis mutant strain [Elektronische Daten] [P De Wulf, W Soetaert, D Schwengers, E Vandamme] WhenBacillus subtilis strain ATCC 21951, a transketolase-deficientd-ribose-producing mutant, was grown ond-glucose plus a second substrate which is metabolized via the oxidative pentose phosphate cycle (d-gluconic acid,d-xylose,l-arabinose ord-xylitol),d-glucose did not catabolite repress metabolism of the second carbon source. Thed-ribose yield obtained with the simultaneously converted carbon substrates, significantly exceeded that when onlyd-glucose was used. In addition, the concentration of glycolytic by-products and the fermentation time significantly decreased. Based on these findings, a fermentation process was developed withB. subtilis strain ATCC 21951 in whichd-glucose (100 g L−1) andd-gluconic acid (50 g L−1) were converted into 45 g L−1 ofd-ribose and 7.5 g L−1 of acetoin. A second process, based ond-glucose andd-xylose (100 g L−1 each), yielded 60 g L−1 ofd-ribose and 4 g L−1 of acetoin plus 2,3-butanediol. Both mixed carbon source fermentations provide excellent alternatives to the less efficientd-glucose-based processes used so far. Society for Industrial Microbiology, 1996 d -ribose nationallicence Bacillus subtilis nationallicence transketolase nationallicence catabolite repression nationallicence pentose phosphate cycle nationallicence De Wulf P. Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure links 653, B-9000, Ghent, Belgium aut Soetaert W. Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure links 653, B-9000, Ghent, Belgium aut Schwengers D. Pfeifer und Langen, Frankenstraße 25, 41539, Dormagen, Germany aut Vandamme E. Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure links 653, B-9000, Ghent, Belgium aut Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 104-109 0169-4146 17:2<104 1996 17 10295 https://doi.org/10.1007/BF01570052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Wulf P. Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure links 653, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Soetaert W. Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure links 653, B-9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Schwengers D. Pfeifer und Langen, Frankenstraße 25, 41539, Dormagen, Germany aut NATIONALLICENCE 700 1- Vandamme E. Laboratory of Industrial Microbiology and Biocatalysis, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure links 653, B-9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 104-109 0169-4146 17:2<104 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer