caa a22 4500 477105653 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01570056 doi (NATIONALLICENCE)springer-10.1007/BF01570056 Control of microbial souring by nitrate, nitrite or glutaraldehyde injection in a sandstone column [Elektronische Daten] [M Reinsel, J Sears, P Stewart, M McInerney] Microbial souring (production of hydrogen sulfide by sulfate-reducing bacteria, SRB) in crushed Berea sandstone columns with oil field-produced water consortia incubated at 60°C was inhibited by the addition of nitrate (NO3) or nitrite (NO 2 − ). Added nitrate (as nitrogen) at a concentration of 0.71 mM resulted in the production of 0.57-0.71 mM nitrite by the native microbial population present during souring and suppressed sulfate reduction to below detection limits. Nitrate added at 0.36 mM did not inhibit active souring but was enough to maintain inhibition if the column had been previously treated with 0.71 mM or greater. Continuous addition of 0.71-0.86 mM nitrite also completely inhibited souring in the column. Pulses of nitrite were more effective than the same amount of nitrite added continuously. Nitrite was more effective at inhibiting souring than was glutaraldehyde, and SRB recovery was delayed longer with nitrite than with glutaraldehyde. It was hypothesized that glutaraldehyde killed SRB while nitrite provided a long-term inhibition without cell death. Removal of nitrate after as long as 3 months of continuous addition allowed SRB in a biofilm to return to their previous level of activity. Inhibition was achieved with much lower levels of nitrate and nitrite, and at higher temperatures, than noted by other researchers. Society for Industrial Microbiology, 1996 souring nationallicence nitrate nationallicence nitrite nationallicence glutaraldehyde nationallicence inhibition nationallicence Reinsel M. National Science Foundation Engineering Research Center for Biofilm Engineering, Montana State University, 59717, Bozeman, MT, USA aut Sears J. National Science Foundation Engineering Research Center for Biofilm Engineering, Montana State University, 59717, Bozeman, MT, USA aut Stewart P. National Science Foundation Engineering Research Center for Biofilm Engineering, Montana State University, 59717, Bozeman, MT, USA aut McInerney M. Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, OK, USA aut Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 128-136 0169-4146 17:2<128 1996 17 10295 https://doi.org/10.1007/BF01570056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reinsel M. National Science Foundation Engineering Research Center for Biofilm Engineering, Montana State University, 59717, Bozeman, MT, USA aut NATIONALLICENCE 700 1- Sears J. National Science Foundation Engineering Research Center for Biofilm Engineering, Montana State University, 59717, Bozeman, MT, USA aut NATIONALLICENCE 700 1- Stewart P. National Science Foundation Engineering Research Center for Biofilm Engineering, Montana State University, 59717, Bozeman, MT, USA aut NATIONALLICENCE 700 1- McInerney M. Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, OK, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 128-136 0169-4146 17:2<128 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer