caa a22 4500 47710567X CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01570054 doi (NATIONALLICENCE)springer-10.1007/BF01570054 A comparison of carbon/energy and complex nitrogen sources for bacterial sulphate-reduction: potential applications to bioprecipitation of toxic metals as sulphides [Elektronische Daten] [C White, G Gadd] Detailed nutrient requirements were determined to maximise efficacy of a sulphate-reducing bacterial mixed culture for biotechnological removal of sulphate, acidity and toxic metals from waste waters. In batch culture, lactate produced the greatest biomass, while ethanol was more effective in stimulating sulphide production and acetate was less effective. The presence of additional bicarbonate and H2 only marginally stimulated sulphide production. The sulphide output per unit of biomass was greatest using ethanol as substrate. In continuous culture, ethanol and lactate were used directly as efficient substrates for sulphate reduction while acetate yielded only slow growth. Glucose was utilised following fermentation to organic acids and therefore had a deleterious effect on pH. Ethanol was selected as the most efficient substrate due to cost and efficient yield of sulphide. On ethanol, the presence of additional carbon sources had no effect on growth or sulphate reduction in batch culture but the presence of complex nitrogen sources (yeast extract or cornsteep) stimulated both. Cornsteep showed the strongest effect and was also preferred on cost grounds. In continuous culture, cornsteep significantly improved the yield of sulphate reduced per unit of ethanol consumed. These results suggest that the most efficient nutrient regime for bioremediation using sulphate-reducing bacteria required both ethanol as carbon source and cornsteep as a complex nitrogen source. Society for Industrial Microbiology, 1996 sulphate-reducing bacteria nationallicence bioremediation nationallicence complex nitrogen sources nationallicence substrates nationallicence White C. Department of Biological Sciences, University of Dundee, DD1 4HN, Dundee, Scotland, UK aut Gadd G. Department of Biological Sciences, University of Dundee, DD1 4HN, Dundee, Scotland, UK aut Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 116-123 0169-4146 17:2<116 1996 17 10295 https://doi.org/10.1007/BF01570054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- White C. Department of Biological Sciences, University of Dundee, DD1 4HN, Dundee, Scotland, UK aut NATIONALLICENCE 700 1- Gadd G. Department of Biological Sciences, University of Dundee, DD1 4HN, Dundee, Scotland, UK aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 116-123 0169-4146 17:2<116 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer