caa a22 4500 477105688 CHVBK 20180405111552.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01570053 doi (NATIONALLICENCE)springer-10.1007/BF01570053 Isolation of bacterial strains capable of using lupanine, the predominant quinolizidine alkaloid in white lupin, as sole carbon and energy source [Elektronische Daten] [F Santana, A Fialho, I Sá-Correia, J Empis] Seven Gram-negative bacterial strains, capable of using lupanine, the predominant quinolizidine alkaloid in white lupin, as sole carbon and energy source, were isolated from soil in whichLupinus albus andL. luteus had been grown. A metabolic profile system (BIOLOG) identified only three of the seven isolates, two asXanthomonas oryzae pvoryzae E and one asGluconobacter cerinus. The maximum specific growth rates of the seven isolates when incubated at 27°C in a medium containing as sole carbon source 2 g L−1 of lupanine, ranged from 0.05 to 0.13 h−1 and the concentration of dry biomass at the stationary phase ranged from 0.7 to 1.1 g L−1. Unidentified strains IST20B and IST40D exhibited the highest maximum specific growth rates (0.13h−1), removed 99% of the initial lupanine after 30 h of incubation, and the dry biomass yields did not exceed 0.4 g per g lupanine consumed. Strain IST20B is of potential use forL. albus debittering because, after 32 h growth in aqueous extracts ofL. albus, 85% of initial alkaloids were removed while the concentration of soluble protein was only reduced by 8%. Society for Industrial Microbiology, 1996 lupanine nationallicence quinolizidine alkaloids nationallicence BIOLOG nationallicence bacterial isolation nationallicence biodegradation nationallicence lupin debittering nationallicence Santana F. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut Fialho A. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut Sá-Correia I. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut Empis J. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 110-115 0169-4146 17:2<110 1996 17 10295 https://doi.org/10.1007/BF01570053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santana F. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut NATIONALLICENCE 700 1- Fialho A. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut NATIONALLICENCE 700 1- Sá-Correia I. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut NATIONALLICENCE 700 1- Empis J. Laboratório de Engenharia Bioquímica, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1000, Lisboa, Portugal aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 110-115 0169-4146 17:2<110 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer