caa a22 4500 477105726 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01570049 doi (NATIONALLICENCE)springer-10.1007/BF01570049 Production of chitinolytic enzymes from a novel species of Aeromonas [Elektronische Daten] [J Huang, C Chen, Y Su] A bacterial strain secreting potent chitinolytic activity was isolated from shrimp-pond water by enrichment culture using colloidal crab-shell chitin as the major carbon source. The isolated bacterium, designated asAeromonas sp No. 16 exhibited a rod-like morphology with a polar flagellum. Under optimal culture conditions in 500-ml shaker flasks, it produced a chitinolytic activity of 1.4 U ml−1. A slightly higher enzymatic activity of 1.5 U ml−1 was obtained when cultivation was carried out in a 5-liter jar fermentor using a medium containing crystalline chitin as the carbon source. The secretion of the enzyme(s) was stimulated by several organic nitrogenous supplements. Most carbon sources tested (glucose, maltose, N-acetylglucosamine, etc) enhanced cell growth, but they slightly inhibited enzyme secretion. Glucosamine (0.5% w/v) severely inhibited cell growth (16% of the control), but it did not significantly affect enzyme secretion. The production of chitinolytic enzymes was pH sensitive and was enhanced by increasing the concentration of colloidal chitin to 1.5%. The observed chitinolytic activity could be attributed to the presence of β-N-acetylglucosaminidase and chitinase. Chitinase was purified by ammonium sulfate fractionation and preparative gel electrophoresis to three major bands on SDS-PAGE. An in-gel enzymatic activity assay indicated that all three bands possessed chitinase activity. Analysis of the enzymatic products indicated that the purified enzyme(s) hydrolyzed colloidal chitin predominantly to N,N-diacetyl-chitobiose and, to a much lesser extent, the mono-, tri, and tetramer of N-acetylglucosamine, suggesting that they are mainly endochitinases. Society for Industrial Microbiology, 1996 chitin nationallicence chitinase nationallicence Aeromonas sp nationallicence Huang J. Department of Agricultural Chemistry, National Taiwan University, 10617, Taipei, Taiwan, Republic of China aut Chen C. Department of Agricultural Chemistry, National Taiwan University, 10617, Taipei, Taiwan, Republic of China aut Su Y. Department of Agricultural Chemistry, National Taiwan University, 10617, Taipei, Taiwan, Republic of China aut Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 89-95 0169-4146 17:2<89 1996 17 10295 https://doi.org/10.1007/BF01570049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang J. Department of Agricultural Chemistry, National Taiwan University, 10617, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chen C. Department of Agricultural Chemistry, National Taiwan University, 10617, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Su Y. Department of Agricultural Chemistry, National Taiwan University, 10617, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 17/2(1996-08-01), 89-95 0169-4146 17:2<89 1996 17 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer