caa a22 4500 477105750 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01569918 doi (NATIONALLICENCE)springer-10.1007/BF01569918 Internal substrate concentrations during biotransformation of octanoic acid into 2-heptanone by spores of Penicillium roquefortii [Elektronische Daten] [C Larroche, I Besson, J Gros] Internal substrate concentrations were monitored during biotransformation of octanoic acid into 2-heptanone by spores ofPenicillium roquefortii. The fatty acid rapidly enters the spores in its undissociated form, and a Collander-type relation shows that it strongly accumulates in the spore wall and membrane; this accumulation is reversible. The reaction takes place with cytoplasmic substrate concentrations that quickly fall to zero, and the process is limited by octanoic acid penetration into the cells. This entry is accompanied by proton efflux and involves an active transport process with a H+-ATPase system that exhibits Michaelian behavior. The driving force is postulated to be ΔpH, which takes a value set by the initial substrate concentration through the stoichiometry of the H+/octanoic acid exchange. Society for Industrial Microbiology, 1996 spores nationallicence fatty acid nationallicence internal concentrations nationallicence transport nationallicence biotransformation nationallicence Penicillium roquefortii nationallicence Larroche C. Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, F-63177, Aubière Cedex, France aut Besson I. Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, F-63177, Aubière Cedex, France aut Gros J. Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, F-63177, Aubière Cedex, France aut Journal of Industrial Microbiology Springer-Verlag 16/1(1996-01-01), 29-35 0169-4146 16:1<29 1996 16 10295 https://doi.org/10.1007/BF01569918 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01569918 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Larroche C. Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, F-63177, Aubière Cedex, France aut NATIONALLICENCE 700 1- Besson I. Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, F-63177, Aubière Cedex, France aut NATIONALLICENCE 700 1- Gros J. Laboratoire de Génie Chimique Biologique, Université Blaise Pascal, F-63177, Aubière Cedex, France aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/1(1996-01-01), 29-35 0169-4146 16:1<29 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer