caa a22 4500 477105769 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01569923 doi (NATIONALLICENCE)springer-10.1007/BF01569923 Effect of alginate encapsulation and selected disinfectants on survival of and phenanthrene mineralization by Pseudomonas sp UG14Lr in creosote-contaminated soil [Elektronische Daten] [S Weir, M Providenti, H Lee, J Trevors] The survival and phenanthrene-mineralizing ability of free and alginate-encapsulatedPseudomonas sp UG14Lr cells were examined in a creosote-contaminated soil. Alginate encapsulation adversely affected both survival and phenanthrene mineralization. This was postulated to be due to concentration of water-soluble toxic compounds in the alginate beads. Toxicity studies showed that the concentrated water-soluble fraction of the creosote-contaminated soil may be toxic toPseudomonas sp UG14Lr in soil with a low moisture content. Survival of alginate-encapsulated cells improved with increasing soil moisture content. Free cells survived well at a steady population of 108 CFU g−1 dry soil for 28 days in the creosote-contaminated soil. However, phenanthrene mineralization was not improved compared to the uninoculated control. This was attributed to the existence of indigenous phenanthrene-mineralizing microorganisms already present in this contaminated soil. The effect of calcium hypochlorite and Germiphene on survival of and phenanthrene mineralization by free and alginate-encapsulatedPseudomonas sp UG14Lr cells in creosote-contaminated soil was also studied. Addition of 0.1% (w/w dry soil) calcium hypochlorite reduced the introduced free cells to below detection limits (10 CFU g−1 dry soil) within 14 days, while Germiphene had no effect on cell numbers. Phenanthrene mineralization by free cells was not adversely affected by treatment with calcium hypochlorite or Germiphene. Survival of alginate-encapsulated cells after treatment with disinfectants was as poor as that without disinfection. The results show that alginate encapsulation may not be a suitable formulation for introduction ofPseudomonas sp UG14Lr into creosote-contaminated soils. Society for Industrial Microbiology, 1996 alginate-encapsulated cells nationallicence Pseudomonas nationallicence soil nationallicence survival nationallicence biodegradation nationallicence Weir S. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Providenti M. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Lee H. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Trevors J. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Journal of Industrial Microbiology Springer-Verlag 16/1(1996-01-01), 62-67 0169-4146 16:1<62 1996 16 10295 https://doi.org/10.1007/BF01569923 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01569923 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weir S. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Providenti M. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Lee H. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Trevors J. Department of Environmental Biology, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/1(1996-01-01), 62-67 0169-4146 16:1<62 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer