caa a22 4500 477105823 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01569919 doi (NATIONALLICENCE)springer-10.1007/BF01569919 Measurement of hydrocarbon-degrading microbial populations by a 96-well plate most-probable-number procedure [Elektronische Daten] [J Haines, B Wrenn, E Holder, K Strohmeier, R Herrington, A Venosa] A 96-well microtiter plate most-probable-number (MPN) procedure was developed to enumerate hydrocarbondegrading microorganisms. The performance of this method, which uses number 2 fuel oil (F2) as the selective growth substrate and reduction of iodonitrotetrazolium violet (INT) to detect positive wells, was evaluated by comparison with an established 24-well microtiter plate MPN procedure (the Sheen Screen), which uses weathered North Slope crude oil as the selective substrate and detects positive wells by emulsification or dispersion of the oil. Both procedures gave similar estimates of the hydrocarbon-degrader population densities in several oil-degrading enrichment cultures and sand samples from a variety of coastal sites. Although several oils were effective substrates for the 96-well procedure, the combination of F2 with INT was best, because the color change associated with INT reduction was more easily detected in the small wells than was disruption of the crude oil slick. The method's accuracy was evaluated by comparing hydrocarbon-degrader MPNs with heterotrophic plate counts for several pure and mixed cultures. For some organisms, it seems likely that a single cell cannot initiate sufficient growth to produce a positive result. Thus, this and other hydrocarbon-degrader MPN procedures might underestimate the hydrocarbon-degrading population, even for culturable organisms. Society for Industrial Microbiology, 1996 MPN nationallicence hydrocarbon-degraders nationallicence crude oil nationallicence tetrazolium nationallicence INT nationallicence Haines J. National Risk Management Research Laboratory, US Environmental Protection Agency, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut Wrenn B. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut Holder E. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut Strohmeier K. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut Herrington R. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut Venosa A. National Risk Management Research Laboratory, US Environmental Protection Agency, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut Journal of Industrial Microbiology Springer-Verlag 16/1(1996-01-01), 36-41 0169-4146 16:1<36 1996 16 10295 https://doi.org/10.1007/BF01569919 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01569919 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haines J. National Risk Management Research Laboratory, US Environmental Protection Agency, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Wrenn B. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Holder E. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Strohmeier K. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Herrington R. Department of Civil and Environmental Engineering, University of Cincinnati, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut NATIONALLICENCE 700 1- Venosa A. National Risk Management Research Laboratory, US Environmental Protection Agency, 26 W Martin Luther King Dr, 45268, Cincinnati, OH, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/1(1996-01-01), 36-41 0169-4146 16:1<36 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer