caa a22 4500 477105882 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01570112 doi (NATIONALLICENCE)springer-10.1007/BF01570112 Electrotransformation of Clostridium thermosaccharolyticum [Elektronische Daten] [T Klapatch, M Guerinot, L Lynd] Transformation of the thermophileClostridium thermosaccharolyticum ATCC 31960 was achieved using plasmid pCTC1 and electroporation. Evidence supporting transformation was provided by Southern blots, detection of the plasmid in 10 out of 10 erythromycin-resistant clones, retransformation ofE. coli andC. thermosaccharolyticum with plasmid DNA isolated fromC. thermosaccharolyticum, and a proportional relationship between the number of transformants and the amount of DNA added. Transformation efficiencies were very low for plasmid DNA prepared fromE. coli (0.6 transformants mg−1 DNA), although somewhat higher for plasmid DNA prepared fromC. thermosaccharolyticum (52 transformants mg−1 DNA). Transformation-dependent erythromycin resistance indicates that an adenosine methylase gene originating fromEnterococcus faecalis, a mesophile, is expressed inC. thermosaccharolyticum. The plasmid pCTC1 appears to be replicated independently of the chromosome, as indicated by visualization of recovered plasmid on gels, and retransformation using recovered plasmid. pCTC1 is maintained inC. thermosaccharolyticum at both 45 and 60°C. Restriction analysis showed little or no rearrangement occurred upon passage through the thermophile. Society for Industrial Microbiology, 1996 transformation nationallicence electroporation nationallicence Clostridium thermosaccharolyticum nationallicence thermophile nationallicence Klapatch T. Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA aut Guerinot M. Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA aut Lynd L. Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA aut Journal of Industrial Microbiology Springer-Verlag 16/6(1996-06-01), 342-347 0169-4146 16:6<342 1996 16 10295 https://doi.org/10.1007/BF01570112 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570112 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klapatch T. Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA aut NATIONALLICENCE 700 1- Guerinot M. Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA aut NATIONALLICENCE 700 1- Lynd L. Department of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/6(1996-06-01), 342-347 0169-4146 16:6<342 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer