caa a22 4500 477105890 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01570115 doi (NATIONALLICENCE)springer-10.1007/BF01570115 The effect of novobiocin on solvent production by Clostridium acetobutylicum [Elektronische Daten] [J Wong, G Bennett] Cells ofClostridium acetobutylicum treated with novobiocin, a DNA gyrase inhibitor, produced higher butyrate levels and lower solvent levels with acetone being the most affected. Seven enzyme activities involved in acid and solvent production were analyzed. Among them, only CoA transferase, required for acetone formation and acid uptake, experienced a significant decrease in activity. As inEscherichia coli andBacillus subtilis, DNA fromC. acetobutylicum became less negatively supercoiled in the early stationary phase (solventogenic stage), as shown by analysis of linking number of a reporter plasmid by agarose gel electrophoresis in the presence of chloroquine. Society for Industrial Microbiology, 1996 supercoiling nationallicence enzymes nationallicence acetone nationallicence butanol nationallicence Clostridium acetobutylicum nationallicence Wong J. Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, 77005-1892, Houston, TX, USA aut Bennett G. Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, 77005-1892, Houston, TX, USA aut Journal of Industrial Microbiology Springer-Verlag 16/6(1996-06-01), 354-359 0169-4146 16:6<354 1996 16 10295 https://doi.org/10.1007/BF01570115 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570115 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wong J. Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, 77005-1892, Houston, TX, USA aut NATIONALLICENCE 700 1- Bennett G. Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, 77005-1892, Houston, TX, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/6(1996-06-01), 354-359 0169-4146 16:6<354 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer