caa a22 4500 477105939 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01570120 doi (NATIONALLICENCE)springer-10.1007/BF01570120 Flagellar proteins of motile spores of Actinomycetes [Elektronische Daten] [N Vesselinova, J Ensign] Flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by SDS-PAGE analysis to flagellins of cells of other bacteria. Several species ofActinoplanes have a major flagellar protein of subunit sizes of 42-43 kDa and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kDa. The flagellar protein sizes of other actinoplanetes ranged from 32-43 kDa (major) and 48-58 kDa (minor). Antibodies formed against the 42-kDa protein ofA. rectilineatus showed cross-reactivity in Western blots against flagellar proteins of spores of otherActinoplanes species, two species ofDactylosporangium and anAmpullariella species. Cross-reactivity was also observed with motile cells of two other actinomycetes,Arthrobacter atrocyaneus and aGeodermatophilus species, and withBacillus subtilis. No cross-reactivity was observed withEscherichia coli orPlanomonospora parontospora flagellar proteins. The amino acid composition and partial N-terminal sequence of the 42-kDa flagellar protein ofA. rectilineatus was compared to literature data for other bacterial flagellins and found to be most similar toB. subtilis 168. Society for Industrial Microbiology, 1996 motile spores nationallicence flagellar proteins of Actinomycetes nationallicence Vesselinova N. Department of Bacteriology, University of Wisconsin, 53706, Madison, Wisconsin, USA aut Ensign J. Department of Bacteriology, University of Wisconsin, 53706, Madison, Wisconsin, USA aut Journal of Industrial Microbiology Springer-Verlag 16/6(1996-06-01), 377-382 0169-4146 16:6<377 1996 16 10295 https://doi.org/10.1007/BF01570120 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570120 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vesselinova N. Department of Bacteriology, University of Wisconsin, 53706, Madison, Wisconsin, USA aut NATIONALLICENCE 700 1- Ensign J. Department of Bacteriology, University of Wisconsin, 53706, Madison, Wisconsin, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/6(1996-06-01), 377-382 0169-4146 16:6<377 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer