caa a22 4500 477106005 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01569997 doi (NATIONALLICENCE)springer-10.1007/BF01569997 Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter [Elektronische Daten] [R Donovan, C Robinson, B Glick] This review examines factors which influence the expression of foreign proteins inEscherichia coli under the transcriptional control of thelac andtac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-β-d-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed. Society for Industrial Microbiology, 1996 lac promoter nationallicence tac promoter nationallicence recombinant DNA nationallicence protein overexpression nationallicence fermentation strategies nationallicence IPTG nationallicence lactose nationallicence Donovan R. Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Robinson C. Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Glick B. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Journal of Industrial Microbiology Springer-Verlag 16/3(1996-03-01), 145-154 0169-4146 16:3<145 1996 16 10295 https://doi.org/10.1007/BF01569997 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01569997 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donovan R. Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 700 1- Robinson C. Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 700 1- Glick B. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/3(1996-03-01), 145-154 0169-4146 16:3<145 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer