caa a22 4500 477106013 CHVBK 20180405111553.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01570003 doi (NATIONALLICENCE)springer-10.1007/BF01570003 Biosorption of cadmium, cobalt, nickel, and strontium by a Bacillus simplex strain isolated from the vadose zone [Elektronische Daten] [N Valentine, H Bolton Jr, M Kingsley, G Drake, D Balkwill, A Plymale] A subsurface Gram-positive, endospore-forming, filamentous bacterium, designated ZAN-044, was isolated from a depth of 96.2 m in the vadose zone of the Hanford Site in Washington State. A phylogenetic analysis of the 16S rRNA gene sequence of strain ZAN-044 revealed it to be 99.5% similar toBacillus simplex strain DSM 1321, indicating that they may be members of the same species.B. simplex ZAN-044 was studied along withBacillus subtilis 168, andEscherichia coli K-12 (AB264), two well-characterized metal-sorbing bacteria, for the binding of Cd2+, Co2+, Ni2+, and Sr2+. There was rapid (less than 1 h) uptake of 1 μM metal by the three bacteria in the order Cd>Ni≥Co>Sr. Binding followed a saturation isotherm at cation concentrations from 0.1 μM to 1 mM. Cation binding was pH-dependent, with less binding at low pH.B. simplex ZAN-044 bound more metal thanB. subtilis orE. coli, demonstrating that subsurface microorganisms can remove significant quantities of metals from solution and may be able to influence radionuclide and metal transport in the subsurface. Society for Industrial Microbiology, 1996 biosorption nationallicence subsurface bacterium nationallicence vadose nationallicence cadmium nationallicence cobalt nationallicence nickel nationallicence strontium nationallicence Valentine N. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut Bolton Jr H. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut Kingsley M. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut Drake G. Florida State University, Tallahassee, FL, USA aut Balkwill D. Florida State University, Tallahassee, FL, USA aut Plymale A. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut Journal of Industrial Microbiology Springer-Verlag 16/3(1996-03-01), 189-196 0169-4146 16:3<189 1996 16 10295 https://doi.org/10.1007/BF01570003 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valentine N. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut NATIONALLICENCE 700 1- Bolton Jr H. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut NATIONALLICENCE 700 1- Kingsley M. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut NATIONALLICENCE 700 1- Drake G. Florida State University, Tallahassee, FL, USA aut NATIONALLICENCE 700 1- Balkwill D. Florida State University, Tallahassee, FL, USA aut NATIONALLICENCE 700 1- Plymale A. Pacific Northwest National Laboratory, 900 Battelle Blvd, Mail stop K4-06, 99352, Richland, WA, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/3(1996-03-01), 189-196 0169-4146 16:3<189 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer