caa a22 4500 477106080 CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01570026 doi (NATIONALLICENCE)springer-10.1007/BF01570026 A method for the selective isolation of Myxococcus directly from soil [Elektronische Daten] [J Karwowski, G Sunga, S Kadam, J McAlpine] A new method is described for the selective isolation of species ofMyxococcus directly from soil by dilution plating. The method involves suppression of competing microorganisms with antibiotics combined with air drying and wet heat treatment of soils. Fungi were eliminated by supplementing the plating medium with cycloheximide and nystatin. Non-sporulating bacteria were controlled by air drying soils and then heating aqueous soil dilutions for 10 min at 56°C. The predominant sporulating bacteria in soil,Streptomyces andBacillus, were suppressed by adding either tiacumicin B, ristocetin or vancomycin to the medium. Swarming ofMyxococcus colonies was controlled with a casein digest-yeast extract plating medium (CY-C10 agar). Ultrasound treatment of soil suspensions gave the highest number ofMyxococcus colonies in the soils studied, but these cultures could be recovered without ultrasound. Strains ofMyxococccus fulvus, M. xanthus, M. coralloides, M. stipitatus andM. virescens were isolated from soil using this technique. Soils examined yielded one or twoMyxococcus species per sample. Society for Industrial Microbiology, 1996 myxobacteria nationallicence Myxococcus nationallicence soil nationallicence selective isolation nationallicence direct isolation method nationallicence Karwowski J. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut Sunga G. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut Kadam S. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut McAlpine J. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut Journal of Industrial Microbiology Springer-Verlag 16/4(1996-04-01), 230-236 0169-4146 16:4<230 1996 16 10295 https://doi.org/10.1007/BF01570026 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570026 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karwowski J. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut NATIONALLICENCE 700 1- Sunga G. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut NATIONALLICENCE 700 1- Kadam S. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut NATIONALLICENCE 700 1- McAlpine J. Natural Product Discovery, Pharmaceutical Products Research and Development, Abbott Laboratories, 60064-3500, Abbott Park, Illinois, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/4(1996-04-01), 230-236 0169-4146 16:4<230 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer