caa a22 4500 477106129 CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01570023 doi (NATIONALLICENCE)springer-10.1007/BF01570023 Initial oxidative and subsequent conjugative metabolites produced during the metabolism of phenanthrene by fungi [Elektronische Daten] [R Casillas, S Crow Jr, T Heinze, J Deck, C Cerniglia] Three filamentous fungi were examined for the ability to biotransform phenanthrene to oxidative (phase I) and conjugative (phase II) metabolites. Phenanthrene metabolites were purified by high-performance liquid chromatography (HPLC) and identified by UV/visible absorption, mass, and1H NMR spectra.Aspergillus niger ATCC 6275,Syncephalastrum racemosum UT-70, andCunninghamella elegans ATCC 9245 initially transformed [9-14C]phenanthrene to produce metabolites at the 9,10-, 1,2-, and 3,4- positions. Subsequently, sulfate conjugates of phase I metabolites were formed byA. niger, S. racemosum, andC. elegans. Minor glucuronide conjugates of 9-phenanthrol and phenanthrenetrans-9,10-dihydrodiol were formed byS. racemosum andA. niger, respectively. In addition,C. elegans produced the glucose conjugates 1-phenanthryl β-d-glucopyranoside and 2-hydroxy-1-phenanthryl β-d-glucopyranoside, a novel metabolite. [9-14C]Phenanthrene metabolites were not detected in organic extracts from biotransformation experiments with the yeasts,Candida lipolytica 37-1,Candida tropicalis ATCC 32113, andCandida maltosa R-42. Society for Industrial Microbiology, 1996 phenanthrene nationallicence fungi nationallicence degradation nationallicence conjugates nationallicence metabolism nationallicence Casillas R. Department of Biology, Georgia State University, 30303, Atlanta, Georgia, USA aut Crow Jr S. Department of Biology, Georgia State University, 30303, Atlanta, Georgia, USA aut Heinze T. National Center for Toxicological Research, Food and Drug Administration, 72079, Jefferson, Arkansas, USA aut Deck J. National Center for Toxicological Research, Food and Drug Administration, 72079, Jefferson, Arkansas, USA aut Cerniglia C. National Center for Toxicological Research, Food and Drug Administration, 72079, Jefferson, Arkansas, USA aut Journal of Industrial Microbiology Springer-Verlag 16/4(1996-04-01), 205-215 0169-4146 16:4<205 1996 16 10295 https://doi.org/10.1007/BF01570023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Casillas R. Department of Biology, Georgia State University, 30303, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Crow Jr S. Department of Biology, Georgia State University, 30303, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Heinze T. National Center for Toxicological Research, Food and Drug Administration, 72079, Jefferson, Arkansas, USA aut NATIONALLICENCE 700 1- Deck J. National Center for Toxicological Research, Food and Drug Administration, 72079, Jefferson, Arkansas, USA aut NATIONALLICENCE 700 1- Cerniglia C. National Center for Toxicological Research, Food and Drug Administration, 72079, Jefferson, Arkansas, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/4(1996-04-01), 205-215 0169-4146 16:4<205 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer