caa a22 4500 477106137 CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01570030 doi (NATIONALLICENCE)springer-10.1007/BF01570030 Strain improvement of Arthrobacter simplex by protoplast fusion [Elektronische Daten] [W-H Liu, L-W Chow, C-K Lo] Anl-tryptophan auxotroph and milky mutants were derived from an inducible cholesterol oxidase-producing bacterium,Arthrobacter simplex USA18, via UV-mutagenesis. Protoplasts of these mutants and a constitutive cholesterol oxidase producer, strain US3011, were prepared by growing cells in the presence of ampicillin (20μg ml−1) followed by digestion with lysozyme. Protoplast fusion between tested strains with complementary characteristics was achieved in the presence of 20-40% polyethylene glycol 6000. The fusion frequency was about 1.5-1.7×10−3. The cholesterol oxidase activity of four fusants in a cholesterol-containing medium was 20-60% higher than that of parental strains. This study demonstrated that protoplast fusion is applicable to strain improvement ofArthrobacter strains for enzyme production. Society for Industrial Microbiology, 1996 protoplast fusion nationallicence protoplasting nationallicence cholesterol oxidase nationallicence Arthrobacter simplex nationallicence Liu W-H Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China aut Chow L-W Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China aut Lo C-K Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China aut Journal of Industrial Microbiology Springer-Verlag 16/4(1996-04-01), 257-260 0169-4146 16:4<257 1996 16 10295 https://doi.org/10.1007/BF01570030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu W-H Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chow L-W Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Lo C-K Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/4(1996-04-01), 257-260 0169-4146 16:4<257 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer