caa a22 4500 47710620X CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01570037 doi (NATIONALLICENCE)springer-10.1007/BF01570037 Seed stage development for improved fermentation performance: Increased milbemycin production by Streptomyces hygroscopicus [Elektronische Daten] [S Warr, C Gershater, S Box] Fermentation development for improved culture productivity can be achieved in a number of ways. Conventional approaches usually concentrate initially on optimisation of the final stage fermentation. However an understanding of the seed stage and its further development can lead to an improvement in final stage productivity. A significant increase in the production of milbemycin VM44866 byStreptomyces hygroscopicus was achieved by manipulation of several factors associated with the seed stage fermentation. Juvenile seeds and seed media containing reduced levels of carbohydrates overcame the detrimental effects of passaging and seed age associated with the standard (control) process. The effect of final stage inoculum level was seed medium-dependent and seed fermentation incubation temperature also affected subsequent milbemycin VM44866 production. These findings were extended to a second milbemycin-producing strain and these results have demonstrated the potential benefits of seed stage optimisation for improved final stage production. Society for Industrial Microbiology, 1996 milbemycin nationallicence inoculum development nationallicence seed stage fermentation nationallicence Streptomyces hygroscopicus nationallicence Warr S. SmithKline Beecham, Brockham Park, RH3 7AJ, Betchworth, Surrey, UK aut Gershater C. SmithKline Beecham, Yew Tree Bottom Road, KT18 5XQ, Epsom, Surrey, UK aut Box S. SmithKline Beecham, Brockham Park, RH3 7AJ, Betchworth, Surrey, UK aut Journal of Industrial Microbiology Springer-Verlag 16/5(1996-05-01), 295-300 0169-4146 16:5<295 1996 16 10295 https://doi.org/10.1007/BF01570037 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570037 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Warr S. SmithKline Beecham, Brockham Park, RH3 7AJ, Betchworth, Surrey, UK aut NATIONALLICENCE 700 1- Gershater C. SmithKline Beecham, Yew Tree Bottom Road, KT18 5XQ, Epsom, Surrey, UK aut NATIONALLICENCE 700 1- Box S. SmithKline Beecham, Brockham Park, RH3 7AJ, Betchworth, Surrey, UK aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/5(1996-05-01), 295-300 0169-4146 16:5<295 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer