caa a22 4500 477106234 CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01570034 doi (NATIONALLICENCE)springer-10.1007/BF01570034 Choice of microbial host for the naphthalene dioxygenase bioconversion [Elektronische Daten] [D. Wilkinson, J Ward, J Woodley] The use of whole cell biotransformations for single and multistep enzyme conversions is gaining widespread application. In this study the naphthalene dioxygenasenah A gene was transferred intoPseudomonas aeruginosa PAC 1R,Escherichia coli JM107 andPseudomonas putida PpG 277. The effect of ethanol on these genetically engineered Gram-negative bacteria was studied by measurement of enzyme activity, stability and cell integrity. Ethanol has been used in biotransformations as a co-substrate carbon source for co-factor recycling and as a co-solvent increasing dissolved substrate and product levels. Ethanol increased the dissolved substrate (naphthalene) concentration slightly and dissolved product ((+)-cis-(1R, 2S)-dihydroxy-1,2-dihydronaphthalene) by approximately 30% at 4% (w/v) ethanol. BothP. aeruginosa PAC 1R andP. putida PpG 277 showed decreased activity with increasing ethanol concentration whilstE. coli enzyme activity increased with increasing ethanol concentration being comparable to that when glucose was used as a carbon source. This project highlighted the many factors involved in the selection of microbial hosts for whole cell biotransformation processes. Society for Industrial Microbiology, 1996 bioconversion nationallicence ethanol nationallicence naphthalene dioxygenase nationallicence co-solvent nationallicence microbial host nationallicence Wilkinson D. The Advanced Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK aut Ward J. Department of Biochemistry and Molecular Biology, University College London, London, UK aut Woodley J. The Advanced Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK aut Journal of Industrial Microbiology Springer-Verlag 16/5(1996-05-01), 274-279 0169-4146 16:5<274 1996 16 10295 https://doi.org/10.1007/BF01570034 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570034 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilkinson D. The Advanced Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK aut NATIONALLICENCE 700 1- Ward J. Department of Biochemistry and Molecular Biology, University College London, London, UK aut NATIONALLICENCE 700 1- Woodley J. The Advanced Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/5(1996-05-01), 274-279 0169-4146 16:5<274 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer