caa a22 4500 477106269 CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01570036 doi (NATIONALLICENCE)springer-10.1007/BF01570036 Biological potential of fungal inocula for bioaugmentation of contaminated soils [Elektronische Daten] [D Leštan, M Leštan, J Chapelle, R Lamar] The suitability of the fluorescein diacetate hydrolyzing activity (FDA) assay for determining the biological potential (ie fungal biomass produced per unit of substrate) of solid pelleted fungal inoculum intended for use in the bioaugmentation of contaminated soils with white-rot fungi, was evaluated. FDA activity of the white-rot fungusPhanerochaete chrysosporium grown on pelleted substrates and on agar was found to be proportional to quantities of fungal ergesterol and fungal dry matter, respectively. Inoculum biological potential was found to be greatly influenced by substrate formulation and structure, and temperature. Biological potential and the type of carrier influenced the ability ofP. chrysosporium to tolerate pentachlorophenol (PCP).Phanerochaete chrysosporium andTrametes versicolor introduced into PCP-contaminated soil on pellets with higher biological potential and higher nitrogen content (C:N ratio of 50∶1), did not remove PCP more efficiently than when the fungi were introduced on pellets with a lower biological potential (C:N ratio of 309∶1). However, under the latter conditions most of the PCP was transformed to pentachloroanisole (PCA). In soil inoculated withT. versicolor on pellets with high biological potential, higher manganese peroxidase activity was detected compared to soil inoculated with pellets with a lower biological potential. Society for Industrial Microbiology, 1996 fungal inoculum nationallicence bioaugmentation nationallicence biological potential nationallicence pentachlorophenol nationallicence Leštan D. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut Leštan M. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut Chapelle J. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut Lamar R. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut Journal of Industrial Microbiology Springer-Verlag 16/5(1996-05-01), 286-294 0169-4146 16:5<286 1996 16 10295 https://doi.org/10.1007/BF01570036 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570036 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leštan D. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut NATIONALLICENCE 700 1- Leštan M. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut NATIONALLICENCE 700 1- Chapelle J. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut NATIONALLICENCE 700 1- Lamar R. Institute for Microbial and Biochemical Technology, USDA, Forest Products Laboratory, One Gifford Pinchot Drive, 53705-2398, Madison, WI, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/5(1996-05-01), 286-294 0169-4146 16:5<286 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer