caa a22 4500 477106285 CHVBK 20180405111554.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01570073 doi (NATIONALLICENCE)springer-10.1007/BF01570073 Production of an extracellular polysaccharide by Agrobacterium sp DS3 NRRL B-14297 isolated from soil [Elektronische Daten] [C Hou, J Ahlgren, W Brown, J Nicholson] A bacterium isolated from soil and identified asAgrobacterium sp produced a water-soluble extracellular polysaccharide capable of producing highly viscous solutions. Gas chromatographic analysis revealed a sugar composition of glucose, galactose and mannose in the molar ratio of 7.5∶2.4∶1, together with 3.7% (w/w) pyruvic acid. Methylation analyses showed the presence of (1→3)-, (1→4)- and (1→6)-linked glucose, (1→3)- and (1→4, 1→6)-linked galactose and a small portion of (1→3)-linked mannose residues. Succinic acid was not present. The molecular weight of the polysaccharide was estimated by light scattering to be 2×106 Da. The viscosity of solutions containing the polysaccharide remained constant from pH 3 to 11, and decreased by 50% when heated from 5 to 55°C. Maximum yield of the polysaccharide, 20 g L−1, was reached in 48 h at 30°C incubation. Society for Industrial Microbiology, 1996 extracellular polysaccharide nationallicence Agrobacterium nationallicence viscous polysaccharide nationallicence Hou C. Oil Chemical Research, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut Ahlgren J. Biopolymer Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut Brown W. Oil Chemical Research, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut Nicholson J. Biopolymer Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut Journal of Industrial Microbiology Springer-Verlag 16/2(1996-02-01), 129-133 0169-4146 16:2<129 1996 16 10295 https://doi.org/10.1007/BF01570073 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01570073 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hou C. Oil Chemical Research, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Ahlgren J. Biopolymer Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Brown W. Oil Chemical Research, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Nicholson J. Biopolymer Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology Springer-Verlag 16/2(1996-02-01), 129-133 0169-4146 16:2<129 1996 16 10295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer