caa a22 4500 477107028 CHVBK 20180405111556.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01412317 doi (NATIONALLICENCE)springer-10.1007/BF01412317 Alterations of local cerebral blood flow, phorbol 12,13-dibutyrate binding activity, and histological damage during acute focal ischaemia in rat brain [Elektronische Daten] A pathophysiology of acute focal ischaemia: Part 1 [N. Inoue, Y. Yamamoto, T. Nagao, S. Goto, S. Nagahiro, Y. Ushio] Summary: The alterations of the local cerebral blood flow (LCBF),3 H-phorbol 12,13-dibutyrate (PDBu) binding activity were measured, and histological findings were also examined during the closed time course (0, 1, 3, 5, 7 hour) after middle cerebral artery occlusion (MCAO) in rat brain to assess the complex pathophysiology of acute focal ischaemia. From 1 to 3 hours after the start of MCAO, significant (p < 0.01) hyperreactivity of the second messenger system involving PDBu binding may be present, despite low perfusion of LCBF, and severe damage in the striatum whereas sparing almost completely the cortex on histological examination. At 5 hours, the PDBu binding activity increased slightly but not significantly but is reduced markedly at 7 hours after MCAO compared with the control group. The measurement of PDBu binding activity, additionally to measuring the LCBF and observation of the histological change might be a useful indicator in determining the threshold and duration of ischaemia which cause functionally irreversible cell damage in the brain. Springer-Verlag, 1996 Cerebral ischaemia nationallicence local cerebral blood flow nationallicence ischaemic damage nationallicence PDBu binding activity nationallicence duration of middle cerebral artery occlusion nationallicence Inoue N. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut Yamamoto Y. Neuro-Isotope Laboratory and Cone Laboratory for Neurosurgical Research, Montreal Neurological Institute, Montreal, Quebec, Canada aut Nagao T. Neuro-Isotope Laboratory and Cone Laboratory for Neurosurgical Research, Montreal Neurological Institute, Montreal, Quebec, Canada aut Goto S. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut Nagahiro S. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut Ushio Y. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut Acta Neurochirurgica Springer-Verlag 138/9(1996-09-01), 1118-1125 0001-6268 138:9<1118 1996 138 701 https://doi.org/10.1007/BF01412317 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01412317 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inoue N. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut NATIONALLICENCE 700 1- Yamamoto Y. Neuro-Isotope Laboratory and Cone Laboratory for Neurosurgical Research, Montreal Neurological Institute, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Nagao T. Neuro-Isotope Laboratory and Cone Laboratory for Neurosurgical Research, Montreal Neurological Institute, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Goto S. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut NATIONALLICENCE 700 1- Nagahiro S. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut NATIONALLICENCE 700 1- Ushio Y. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto, Japan aut NATIONALLICENCE 773 0- Acta Neurochirurgica Springer-Verlag 138/9(1996-09-01), 1118-1125 0001-6268 138:9<1118 1996 138 701 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer