caa a22 4500 477107370 CHVBK 20180405111557.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF01411124 doi (NATIONALLICENCE)springer-10.1007/BF01411124 Matrix metalloproteinase-9 secretion by human pituitary adenomas detected by cell immunoblot analysis [Elektronische Daten] [H. Kawamoto, K. Kawamoto, T. Mizoue, T. Uozumi, K. Arita, K. Kurisu] Summary: Twenty-two pituitary adenomas were examined on the secretion of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) using a cell immunoblot assay, and discussed regarding an association between cavernous sinus invasion and the secretion of these proteins. The cell immunoblot assay, a kind of immunoblot procedure, is able to detect proteins at the single cell level and to detect the incidence of tumour cells secreting the target proteins in the total tumour cell population. The incidence of tumour cells secreting MMP-9 was significantly higher in invasive adenomas than in noninvasive ones. On the other hand, TIMP-1 secretion was not detected in any adenomas in this study. This result suggested that MMP-9 secretion, and especially the number of MMP-9-secreting cells, may be associated with cavernous sinus invasion of pituitary adenomas. Springer-Verlag, 1996 Matrix metalloproteinase-9 nationallicence pituitary adenomas nationallicence cavernous sinus invasion nationallicence cell immunoblot nationallicence Kawamoto H. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut Kawamoto K. Department of Biology, Toyama University Faculty of Biology, Toyama, Japan aut Mizoue T. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut Uozumi T. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut Arita K. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut Kurisu K. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut Acta Neurochirurgica Springer-Verlag 138/12(1996-12-01), 1442-1448 0001-6268 138:12<1442 1996 138 701 https://doi.org/10.1007/BF01411124 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01411124 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawamoto H. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut NATIONALLICENCE 700 1- Kawamoto K. Department of Biology, Toyama University Faculty of Biology, Toyama, Japan aut NATIONALLICENCE 700 1- Mizoue T. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut NATIONALLICENCE 700 1- Uozumi T. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut NATIONALLICENCE 700 1- Arita K. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut NATIONALLICENCE 700 1- Kurisu K. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima aut NATIONALLICENCE 773 0- Acta Neurochirurgica Springer-Verlag 138/12(1996-12-01), 1442-1448 0001-6268 138:12<1442 1996 138 701 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer