caa a22 4500 477107753 CHVBK 20180405111600.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF01809753 doi (NATIONALLICENCE)springer-10.1007/BF01809753 Development of calcitonin gene-related peptide slow-release tablet implanted in CSF space for prevention of cerebral vasospasm after experimental subarachnoid haemorrhage [Elektronische Daten] [I. Ahmad, S. Imaizumi, H. Shimizu, T. Kaminuma, N. Ochiai, M. Tajima, T. Yoshimoto] Summary: The calcitonin gene-related peptide (CGRP), a known potent intrinsic cerebral vasodilator, is contained in the sensory nerves from trigeminal ganglia that inervate the cerebral arteries. We previously reported that human α CGRP (hCGRP) dilates spastic cerebral arteries after experimental subarachnoid haemorrhage (SAH) in rabbits. In the present study, we investigated the prophylactic potential of a sustained higher cerebrospinal fluid level ofhCGRP against experimental cerebral vasospasm. AnhCGRP slow-release tablet (hCGRP s-r tablet) was developed for cisternal implantation. Experimental SAH was induced by percutaneous cisternal injection of autologous arterial blood. Angiography was initiated on day 1 (before SAH) and performed everyday. ThehCGRP s-r tablet was implanted into the cisterna magna on day 2 in the treated groups. The spastic response of the basilar artery was maximized on day 4 in the non-treated (80.7% of day 1) and the placebo-treated (79.3%) groups. In contrast, the arterial diameters on day 4 were 96.1% and 90.5% of day 1 in the groups implanted withhCGRP 24 μg and 153 μg s-r tablets, respectively. We also measured the concentration ofhCGRP in the cerebrospinal fluid (CSF) following implantation of thehCGRP 24 μg s-r tablet in the cisterna magna. The hCGRP concentration before implantation was below the dectable level. Following implantation, thehCGRP level in the CSF was 23.12 nmol/L on the second day and remained at elevated levels until the fifth day. These experiments suggest that the intrathecal single implantation of thehCGRP s-r tablet could produce an elevated concentration ofhCGRP in the CSF over five days and have prevented the cerebral vasospasm after SAH in the rabbit. ThehCGRP s-r tablet may be clinically applicable in the treatment of patients with SAH against cerebral vasospasm. Springer-Verlag, 1996 Calcitonin gene-related peptide nationallicence slow-release tablet nationallicence subarachnoid haemorrhage nationallicence cerebral vasospasm nationallicence Ahmad I. Department of Neurosurgery, Tohoku University School of Medicine, Sendai aut Imaizumi S. Department of Neurosurgery, Iwate Prefectoral Isawa Hospital, Iwate aut Shimizu H. Department of Neurosurgery, Tohoku University School of Medicine, Sendai aut Kaminuma T. Shiseido Research Centre, Yokohama, Japan aut Ochiai N. Shiseido Research Centre, Yokohama, Japan aut Tajima M. Shiseido Research Centre, Yokohama, Japan aut Yoshimoto T. Department of Neurosurgery, Tohoku University School of Medicine, Sendai aut Acta Neurochirurgica Springer-Verlag 138/10(1996-10-01), 1230-1240 0001-6268 138:10<1230 1996 138 701 https://doi.org/10.1007/BF01809753 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01809753 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahmad I. Department of Neurosurgery, Tohoku University School of Medicine, Sendai aut NATIONALLICENCE 700 1- Imaizumi S. Department of Neurosurgery, Iwate Prefectoral Isawa Hospital, Iwate aut NATIONALLICENCE 700 1- Shimizu H. Department of Neurosurgery, Tohoku University School of Medicine, Sendai aut NATIONALLICENCE 700 1- Kaminuma T. Shiseido Research Centre, Yokohama, Japan aut NATIONALLICENCE 700 1- Ochiai N. Shiseido Research Centre, Yokohama, Japan aut NATIONALLICENCE 700 1- Tajima M. Shiseido Research Centre, Yokohama, Japan aut NATIONALLICENCE 700 1- Yoshimoto T. Department of Neurosurgery, Tohoku University School of Medicine, Sendai aut NATIONALLICENCE 773 0- Acta Neurochirurgica Springer-Verlag 138/10(1996-10-01), 1230-1240 0001-6268 138:10<1230 1996 138 701 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer