caa a22 4500 477107877 CHVBK 20180405111600.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01420307 doi (NATIONALLICENCE)springer-10.1007/BF01420307 Immunohistochemical analysis of the nm23 gene product (NDP kinase) expression in astrocytic neoplasms [Elektronische Daten] [H. Nawashiro, Y. Ozeki, K. Takishima, K. Shima, H. Chigasaki] Summary: The expression levels of nm23-H1 have been reported to correlate with the metastatic potential of some tumours. We have treated a child with a rare case of astrocytoma with diffuse osteoblastic metastases. We therefore decided to examine the expression of the nm23 gene product in 24 gliomas in order to clarify the association of its expression with the clinical features of the disease. A polyclonal antibody against a GST/nm23-H1 fusion protein was raised in rabbits. Twenty-four specimens, including 5 recurrent gliomas and one extraneural metastasis, were obtained from 19 patients treated surgically between 1990 and 1993 in our hospital. Immunohistochemical staining was performed on paraffin sections using an avidin-biotinyl peroxidase complex method. Of the 24 astrocytic neoplasms, 3 (12.5%) specimens from one patient with diffuse bony metastases stained intensely with nm23-H1. Two specimens obtained from glioblastoma multiforme patients stained weakly. The other 19 specimens were negative for nm23-H1 expression. Little or no nm23 expression was observed in adjacent nontumourous cerebral tissues. The results suggest that high levels of nm23 expression might correlate with extraneural metastatic potential in astrocytic neoplasms. Springer-Verlag, 1996 nm23 nationallicence nucleoside diphosphate kinase nationallicence glioma nationallicence immunohistochemistry nationallicence extraneural metastasis nationallicence Nawashiro H. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan aut Ozeki Y. Department of Biochemistry, National Defense Medical College, Tokorozawa, Saitama, Japan aut Takishima K. Department of Biochemistry, National Defense Medical College, Tokorozawa, Saitama, Japan aut Shima K. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan aut Chigasaki H. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan aut Acta Neurochirurgica Springer-Verlag 138/4(1996-04-01), 445-450 0001-6268 138:4<445 1996 138 701 https://doi.org/10.1007/BF01420307 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01420307 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nawashiro H. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Ozeki Y. Department of Biochemistry, National Defense Medical College, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Takishima K. Department of Biochemistry, National Defense Medical College, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Shima K. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Chigasaki H. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 773 0- Acta Neurochirurgica Springer-Verlag 138/4(1996-04-01), 445-450 0001-6268 138:4<445 1996 138 701 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer