caa a22 4500 47710861X CHVBK 20180405111602.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01411367 doi (NATIONALLICENCE)springer-10.1007/BF01411367 Amino-acid release from human cerebral cortex during simulated ischaemia in vitro [Elektronische Daten] [E. Hegstad, J. Berg-Johnsen, T. Haugstad, E. Hauglie-Hanssen, I. Langmoen] Summary: The aim of the present study was to investigate the release of amino-acids in human cerebral cortex during membrane depolarization and simulated ischaemia (energy deprivation). Superfluous tissue from temporal lobe resections for epilepsy was cut into 500 μm thick slices and incubated in vitro. Membrane depolarization with 50 mM K+ caused a release of glutamate, aspartate, GABA and glycine, but not glutamine or leucine. The release of glutamate and GABA was Ca++-dependent. Slices were exposed to simulated ischaemia (energy deprivation; ED) by combined glucose/oxygen deprivation. This caused a Ca++-indepedent release of glutamate, aspartate, GABA, glycine, and taurine which started after 8 min, peaked at the end or shortly after the 27 min period of ED, and returned to control levels within 11 min following termination of ED. Preloaded D-[3H]aspartate was released both during K+-stimulation and ED. Release of D-[3H]aspartate during ED was delayed compared to glutamate supporting an initial phase of synaptic glutamate release. Uptake of L-[3H]glutamate was increased during the period of glutamate release, suggesting passive diffusion across the cell membrane or enhanced transport efficacy in cellular elements with functioning uptake mechanisms. Springer-Verlag, 1996 Cerebral cortex nationallicence amino-acids release nationallicence human nationallicence cerebral ischaemia nationallicence Hegstad E. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut Berg-Johnsen J. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut Haugstad T. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut Hauglie-Hanssen E. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut Langmoen I. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut Acta Neurochirurgica Springer-Verlag 138/2(1996-02-01), 234-241 0001-6268 138:2<234 1996 138 701 https://doi.org/10.1007/BF01411367 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01411367 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hegstad E. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut NATIONALLICENCE 700 1- Berg-Johnsen J. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut NATIONALLICENCE 700 1- Haugstad T. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut NATIONALLICENCE 700 1- Hauglie-Hanssen E. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut NATIONALLICENCE 700 1- Langmoen I. Institute for Surgical Research and Department of Neurosurgery, Rikshospitalet, The National Hospital, University of Oslo, Norway aut NATIONALLICENCE 773 0- Acta Neurochirurgica Springer-Verlag 138/2(1996-02-01), 234-241 0001-6268 138:2<234 1996 138 701 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer