caa a22 4500 477109268 CHVBK 20180405111604.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00029284 doi (NATIONALLICENCE)springer-10.1007/BF00029284 Identification of Agrobacterium and Rhizobium species based on cellular fatty acid composition [Elektronische Daten] [B. Jarvis, S. Sivakumaran, S. Tighe, M. Gillis] The increasing number of phylogenetically defined species in the genera Agrobacterium, Rhizobium and Sinorhizobium suggests a need for a rapid identification method which will distinguish between these species. We have examined 65 strains of Agrobacterium representing: A. tumefacies, (34); A. rhizogenes, (16) A. vitis, (10) A. rubi (2) and some unclassified strains, and 150 strains of Rhizobium and Sinorhizobium representing: R. etli (21); R. galegae (20); R. huakuii (17); R. leguminosarum (20); R. loti (16); R. topici (18); S. fredii (19); and S. meliloti (20). Fatty acid methyl esters (FAME) were obtained from each strain, as previously described, and analysed by gas-chromatography using the MIDI Hewlett-Packard Microbial Identification System (MIS). Fatty acid profiles were recorded, characteristic fatty acids noted and the overall similarities between fatty acid profiles for each species calculated. Relationships between species were also derived from the fatty acid data by principal component analysis. This showed overlapping clusters for strains of R. leguminosarum and R. etli, R. topici and A. rhizogenes and S. fredii and S. meliloti within one supercluster. Strains of A. tumefaciens, A rubi, A. vitis and R. galegae formed a second supercluster while R. loti and R. huakuii strains formed a third cluster well separated from all the other strains. The fatty acid profiles were used to correctly identify at least 94% of the strains representing each species in the collection except R. etli. R. etli strains (23.8%) were misidentified as R. leguminosarum. This was attributed to the high similarity (44.7%) between R. etli and R. leguminosarum. It is concluded that whole cell fatty acid analysis should form part of the polyphasic description of new species of root nodule bacteria, with the proviso that growth conditions and analytical methods be carefully standardized. It is suggested that FAME-MIS system and the database we have compiled provide a basis for future development. Kluwer Academic Publishers, 1996 Agrobacterium nationallicence FAME nationallicence fatty acid analysis nationallicence rapid identification nationallicence Rhizobium nationallicence 16S rRNA : 16S ribosomal ribonucleic acid nationallicence MIS : Microbial identification system nationallicence FAME : Fatty acid methyl ester nationallicence ECL : Equivalent carbon length nationallicence Jarvis B. Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand aut Sivakumaran S. Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand aut Tighe S. Analytical Services Inc., P.O. Box 626, 05453, Essex Junction, VT, USA aut Gillis M. Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B-9000, Ghent, Belgium aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 184/1(1996-03-01), 143-158 0032-079X 184:1<143 1996 184 11104 https://doi.org/10.1007/BF00029284 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00029284 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jarvis B. Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Sivakumaran S. Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Tighe S. Analytical Services Inc., P.O. Box 626, 05453, Essex Junction, VT, USA aut NATIONALLICENCE 700 1- Gillis M. Laboratorium voor Microbiologie, Universiteit Gent, K L Ledeganckstraat 35, B-9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl// 184/1(1996-03-01), 143-158 0032-079X 184:1<143 1996 184 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer