caa a22 4500 477109837 CHVBK 20180405111605.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02257527 doi (NATIONALLICENCE)springer-10.1007/BF02257527 Colonisation patterns of root tissues by Phytophthora nicotianae var. parasitica related to reduced disease in mycorrhizal tomato [Elektronische Daten] [C. Cordier, S. Gianinazzi, V. Gianinazzi-Pearson] Tomato plants pre-colonised by the arbuscular mycorrhizal fungusGlomus mosseae showed decreased root damage by the pathogenPhytophthora nicotianae var.parasitica. In analyses of the cellular bases of their bioprotective effect, a prerequisite for cytological investigations of tissue interactions betweenG. mosseae andP. nicotianae v.parasitica was to discriminate between the hyphae of the two fungi within root tissues. We report the use of antibodies as useful tools, in the absence of an appropriate stain for distinguishing hyphae ofP. nicotianae v.parasitica from those ofG. mosseae inside roots, and present observations on the colonisation patterns by the pathogenic fungus alone or during interactions in mycorrhizal roots. Infection intensity of the pathogen, estimated using an immunoenzyme labelling technique on whole root fragments, was lower in mycorrhizal roots. Immunogold labelling ofP. nicotianae v.parasitica on cross-sections of infected tomato roots showed that inter or intracellular hyphae developed mainly in the cortex, and their presence induced necrosis of host cells, the wall and contents of which showed a strong autofluorescence in reaction to the pathogen. In dual fungal infections of tomato root systems, hyphae of the symbiont and the pathogen were in most cases in different root regions, but they could also be observed in the same root tissues. The number ofP. nicotianae v.parasitica hyphae growing in the root cortex was greatly reduced in mycorrhizal root systems, and in mycorrhizal tissues infected by the pathogen, arbuscule-containing cells surrounded by intercellularP. nicotianae v.parasitica hyphae did not necrose and only a weak autofluorescence was associated with the host cells. Results are discussed in relation to possible processes involved in the phenomenon of bioprotection in arbuscular mycorrhizal plants. Kluwer Academic Publishers, 1996 biocontrol nationallicence G. mosseae nationallicence immunocytochemistry nationallicence P. nicotianae var. parasitica nationallicence tomato nationallicence Cordier C. Laboratoire de Phytoparasitologie INRA/CNRS, SGAP, BV 1540, F-21034, Dijon Cédex, France aut Gianinazzi S. Laboratoire de Phytoparasitologie INRA/CNRS, SGAP, BV 1540, F-21034, Dijon Cédex, France aut Gianinazzi-Pearson V. Laboratoire de Phytoparasitologie INRA/CNRS, SGAP, BV 1540, F-21034, Dijon Cédex, France aut Plant and Soil Kluwer Academic Publishers 185/2(1996-09-01), 223-232 0032-079X 185:2<223 1996 185 11104 https://doi.org/10.1007/BF02257527 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02257527 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cordier C. Laboratoire de Phytoparasitologie INRA/CNRS, SGAP, BV 1540, F-21034, Dijon Cédex, France aut NATIONALLICENCE 700 1- Gianinazzi S. Laboratoire de Phytoparasitologie INRA/CNRS, SGAP, BV 1540, F-21034, Dijon Cédex, France aut NATIONALLICENCE 700 1- Gianinazzi-Pearson V. Laboratoire de Phytoparasitologie INRA/CNRS, SGAP, BV 1540, F-21034, Dijon Cédex, France aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers 185/2(1996-09-01), 223-232 0032-079X 185:2<223 1996 185 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 220019401 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer